RayBio® C-Series Human Angiogenesis Antibody Array 2 Kit. Detects 23 Human Angiogenic Factors. Suitable for all liquid sample types.
Human Angiogenesis Array C2
Human Angiogenesis Array C2
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 23 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Angiogenesis, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Angiopoietin-1
| Angiopoietin-2
| Angiostatin
| Endostatin
| GCSF
|
GM-CSF
| I-309 (TCA-3/CCL1)
| IL-10
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
IL-2
| IL-4
| I-TAC (CXCL11)
| MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
|
MMP-1
| MMP-9
| PECAM-1 (CD31)
| Tie-2
| TNF alpha
|
uPAR
| VEGFR2
| VEGFR3
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Angiogenesis Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Increased Levels of BAMBI Inhibit Canonical TGF-β Signaling in Chronic Wound Tissues
- Species:
- Human
- Sample Type:
- sera, wound tissue
- Year:
- 2023
- Journal Name:
- Cells
Higher Expression of Annexin A2 in Metastatic Bladder Urothelial Carcinoma Promotes Migration and Invasion
- Species:
- Human
- Sample Type:
- T24 cell
- Year:
- 2022
- Journal Name:
- Cancers
miR-143-3p targeting of ITGA6 suppresses tumour growth and angiogenesis by downregulating PLGF expression via the PI3K/AKT pathway in gallbladder carcinoma
- Species:
- Human
- Sample Type:
- Conditioned Media (Gall bladder cancner cell line, NOZ treated with microRNAs)
- Year:
- 2018
- Journal Name:
- Cell Death and Disease
Inflammation and N-formyl peptide receptors mediate the angiogenic activity of human vitreous humour in proliferative diabetic retinopathy
- Species:
- Human
- Sample Type:
- Vitreous Fluid (Proliferative diabetic retinopathy)
- Year:
- 2017
- Journal Name:
- Diabetologia
- PubMed ID:
- 28083635
Crosstalk between bone marrow-derived myofibroblasts and gastric cancer cells regulates cancer stemness and promotes tumorigenesis
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Bone Marrow fibroblasts and MKN28 gastric cancer cell lines)
- Year:
- 2016
- Journal Name:
- Oncogene
Expression of factors involved in the regulation of angiogenesis in the full-term human placenta: Effects of in vitro fertilization
- Species:
- Human
- Sample Type:
- Tissue lysate (Placental Protein, 1mg)
- Year:
- 2016
- Journal Name:
- Reproductive Biology
- PubMed ID:
- 27288334
Mesenchymal Stem Cells Isolated From Human Gliomas Increase Proliferation and Maintain Stemness of Glioma Stem Cells Through the IL-6/gp130/STAT3 Pathway
- Species:
- Human
- Sample Type:
- Conditioned Media (Mesenchymal cells in gliomas help maintain "stemness" in gioma cells)
- Year:
- 2015
- Journal Name:
- Stem Cells
- PubMed ID:
- 25966666
Urinary ADAM12 and MMP-9/NGAL Complex Detect the Presence of Gastric Cancer
- Species:
- Human
- Sample Type:
- Urine (Gastric Cancer patients)
- Year:
- 2015
- Journal Name:
- Cancer Prevention Research
Human endothelial colony forming cells from adult peripheral blood have enhanced sprouting angiogenic potential through up-regulating VEGFR2 signaling
- Species:
- Human
- Sample Type:
- Cell Lysate (HUVEC and Endothelial colony forming cell lysates)
- Year:
- 2015
- Journal Name:
- International Journal of Cardiology
- PubMed ID:
- 26113473
The angiogenic responses induced by release of angiogenic proteins from tumor cell-activated platelets are regulated by distinct molecular pathways
- Species:
- Human
- Sample Type:
- Platelet Releasate (Healthy Patients platelets triggred with tumor cells)
- Year:
- 2015
- Journal Name:
- Iubmb Life
- PubMed ID:
- 26283102