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Human B2M ELISA Kit
Human B2M ELISA Kit
RayBio® Human Beta-2 Microglobulin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 6 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 6 pg/ml - 1000 pg/ml |
| Specificity | Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Adiponectin (ACRP30), Adipsin (Complement Factor D), Alpha-fetoprotein, ANGPTL4, BCAM, CA125, CA15-3, CEA, CRP (C-Reactive Protein), ErbB2, Ferritin, FSH, GRO alpha (CXCL1), HCG beta, IGF-1 R, IL-1 R2, IL-3, IL-18 R beta (AcPL), IL-21, Leptin, MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, NCAM-1 (CD56), Nidogen-1, NSE, Oncostatin M, Procalcitonin, Prolactin, PSA-free, Siglec-9, TACE, Thyroglobulin, TIMP-4, TSH. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 10,000 - 100,000 fold |
| Accession Number | P61769 |
| Gene Id | 567 |
| Gene Symbols | B2M, CDABP0092, HDCMA22P |
| Protein Name / Synonyms | Beta-2-microglobulin [Cleaved into: Beta-2-microglobulin form pI 5.3] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human B2M ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Beta-2 Microglobulin (B2M), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Plasma Mucin-1 as a Potential Biomarker for Diabetic Peripheral Neuropathy in Type 2 Diabetes
- Species:
- Human
- Sample Type:
- Plasma (T2D with/without peripheral neuropathy)
- Year:
- 2026
- Journal Name:
- Biomolecules
- PubMed ID:
- 41594668
Evaluation of beta-2 microglobulin and alpha-2 macroglobulin levels in patients with different periodontal diseases
- Species:
- Human
- Sample Type:
- Gingival Crevicular Fluid (gingival crevicular fluid)
- Year:
- 2013
- PubMed ID:
- 23713636
Urinary biomarkers of chronic allograft nephropathy
- Species:
- Human
- Sample Type:
- Urine (Renal transplant patients undergoing chronic nephropathy)
- Year:
- 2015
- Journal Name:
- Proteomics - Clinical Applications
- PubMed ID:
- 25951805
