Human Free Thyroxine,FT4 ELISA Kit
Cat# CSB-E05078h-96T
Size : 96T
Brand : Cusabio
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Free Thyroxine,FT4
Abbreviation
FT4
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
4 pmol/L-64 pmol/L
Sensitivity
1.08 pmol/L
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 85-99 | ||||||
| 1:2 | Average % | 90 | |||||
| Range % | 87-100 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 88-98 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 86-98 | ||||||
Recovery
| The recovery of human FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-98 | |||||
| EDTA plasma (n=4) | 96 | 93-102 | |||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Human FT4 ELISA Kit was designed for the quantitative measurement of Human FT4 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 4 pmol/L-64 pmol/L and the sensitivity is 1.08 pmol/L.
Citations
- AO AJIBARE,ResearchGate,2021
- OA Ojo, et al,Journal of Endocrinology, Metabolism and Diabetes of South Africa,2019
Customer Reviews and Q&A
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Email: support@cusabio.com
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