Human Poliomyelitis Virus IgG antibody (PV-Ab-IgG) ELISA Kit

This assay employs a two-site sandwich ELISA to quantitate PV-Ab-IgG in samples. An antibody specific for PV-Ab-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PV-Ab-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PV-Ab-IgG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PV-Ab-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.