Reagents and instruments for immunology, cell biology and molecular biology.
 
> IL2 (Interleukin-2, IL-2, T-cell Growth Factor, TCGF, Aldesleukin) (HRP)

IL2 (Interleukin-2, IL-2, T-cell Growth Factor, TCGF, Aldesleukin) (HRP)

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Cat# 143614-HRP-100ul

Size : 100ul

Brand : US Biological


143614-HRP IL2 (Interleukin-2, IL-2, T-cell Growth Factor, TCGF, Aldesleukin) (HRP)

Clone Type
Monoclonal
Host
mouse
Source
human
Isotype
IgG1,k
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 4-8ug/ml of this antibody will detect at least 1000pg/ml of recombinant human IL-2 when used with biotinylated antigen affinity purified anti-human IL-2 as the detection antibody at a concentration of approximately 1-2ug/ml.
Western Blot: To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 1-2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-2 is 2-4ng/lane, under reducing or non-reducing conditions.
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.5ug/ml-5ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: 14G11|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).|Purity: Purified by immunoaffinity chromatography.|Immunogen: Recombinant protein corresponding to human IL-2 expressed in E. coli.|Specificity: Recognizes human IL-2.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant protein corresponding to human IL-2 expressed in E. coli.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human IL-2.