Laminin B2

Applications:
Suitable for use in Immunohistochemistry, Dot Blot and Immunoprecipitation. Not recommended for Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (unfixed, frozen): 10ug/ml using monkey brain sections
Dot Blot: 0.5-2ug/ml detects mouse Laminin in a dot blot assay.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse-rat hybridoma of X63.Ag8 653 mouse myeloma with Fisher rat splenocytes.

Immunohistochemistry
1. 10um unfixed, frozen tissue sections were washed with PBS twice, for 20 minutes.
2. Add 400ul of 8% albumin in PBS and incubate for 1 hour at room temperature.
3. Wash the sections with PBS four times, 15 minutes each, at room temperature.
4. Incubate the sections with a 10ug/ml a-Mouse Laminin B2 Chain in 1.0% albumin in PBS and incubate overnight at 4°C. Also run a negative control (no primary antibody) to check for non-specific staining.
5. Wash the sections with PBS twice for 30 minutes.
6. Incubate the sections with a 1:100 dilution of goat anti-rat IgG fluorescein conjugated secondary antibody in 1% BSA in PBS for 2 hours at room temperature.
7. Wash the section with PBS three times, 15 minutes each.
8. Examine the sections under a fluorescent microscope.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.