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> Maltotetraose [34612-38-9]

Maltotetraose [34612-38-9]

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Cat# HY-N2464-5mg

Size : 5mg

Brand : MedChemExpress


Description

Maltotetraose can serve as a substrate for enzyme-linked assays to measure amylase activity in biological fluids. Maltotetraose has oral active, and reduces TNF-α-induced inflammatory responses by inhibiting NF-κB activity and decreasing ICAM-1 expression. Maltotetraose also inhibits PDGF-induced vascular smooth muscle cell migration and neovascularization. Additionally, Maltotetraose derivatives can function as probes for detecting bacterial infections by targeting the maltodextrin transporter. With good long-term safety, Maltotetraose holds promise for research in atherosclerosis-related diseases[1][2][3][4].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Maltotetraose (20-40 μM, 30 min) suppresses NF-κB activation and ICAM-1 expression, potentially aiding in the early prevention of atherosclerosis[2].
Maltotetraose (20 μM, 30 min) inhibits PDGF-induced vascular smooth muscle cell migration, suggesting a potential role in atherosclerosis prevention[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Maltotetraose Related Antibodies

Western Blot Analysis[2]

Cell Line: MOVAS-1 mouse vascular smooth muscle cells
Concentration: 20-40 μM
Incubation Time: 30 min (Cells were pretreated with Maltotetraose (20-40 μM) for 30 minutes, followed by TNF-α (10 ng/mL) stimulation for 3-8 hours)
Result: Inhibited the phosphorylation of NF-κB-related protein.

RT-PCR[2]

Cell Line: MOVAS-1 mouse vascular smooth muscle cells
Concentration: 20-40 μM
Incubation Time: 30 min (Cells were pretreated with Maltotetraose (20-40 μM) for 30 minutes, followed by TNF-α (10 ng/mL) stimulation for 3-8 hours)
Result: Significantly decreased ICAM-1 mRNA levels.

Cell Migration Assay [2]

Cell Line: MOVAS-1 mouse vascular smooth muscle cells
Concentration: 20 μM
Incubation Time: 30 min (Cells were pretreated with Maltotetraose (20 μM) for 30 minutes, followed by PDGF (20 ng/mL) stimulation for 6-7 days)
Result: Significantly inhibited PDGF-induced vascular smooth muscle cell migration and new blood vessel formation in aortic ring explants.
In Vivo

Maltotetraose (10% in diet or 5,000 mg/kg via p.o., once daily for 90 days) dose not induce significant toxicity, suggesting good long-term safety[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wistar rats[3]
Dosage: Dietary group: 10% (equivalent to 6,818 mg/kg/day in males, 7,464 mg/kg/day in females); Gavage group: 1,000, 3,000, or 5,000 mg/kg/day
Administration: Dietary intake or oral gavage (p.o.), Once daily for 90 consecutive days
Result: Sid not cause mortality or severe adverse effects in rats over 90 days.
Reduced food intake but no significant body weight differences (The 5,000 mg/kg/day gavage group).
Molecular Weight

666.58

Formula

C24H42O21
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H](O[C@H](O[C@@]([C@H](O)CO)(13)[C@H](O)[C@@H](O)C=O)[C@H](O)[C@H]1O)[C@@]1(13)O[C@H]2O[C@@H]([C@@H](O[C@@]3(13)O[C@@H]([C@@H](O)[C@H](O)[C@H]3O)CO)[C@H](O)[C@H]2O)CO
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (187.52 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5002 mL 7.5010 mL 15.0019 mL
5 mM 0.3000 mL 1.5002 mL 3.0004 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (150.02 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References