Mix-n-Stain™ CF®405M Small Ligand Labeling Kit
Cat# 92362
Size : 1kit
Brand : Biotium
Mix-n-Stain™ CF®405M Small Ligand Labeling Kit
Figure 2. Multiplex imaging in live HeLa cells using Mix-n-Stain™-labeled ligands. CF®488A SNAP-tag® ligand was used to detect surface expression of SNAP-ADRβ2 protein (green). Mitochondrial CF®540 CLIP-tag™ ligand was used to detect mitochondrial CLIP-Cox8A protein (red). Nuclei were stained blue with Hoechst 33342.
Figure 3. CLIP-tag™ ligand was labeled with the CF®488A Mix-n-Stain™ Small Ligand Labeling Kit, then used to detect cell surface expression of CLIP-NK1R protein (green). Cells were fixed, permeabilized, stained with CF®633 phalloidin (red), and mounted with EverBrite™ Mounting Medium with DAPI.
Figure 1. Live cell imaging of HeLa cells expressing CLIP-NK1R labeled with (A) CLIP-tag™ ligand conjugated to green fluorescent CF®488A using Mix-n-Stain™ compared to (B) CLIP-surface 488 from New England BioLabs. Cell nuclei were stained blue with Hoechst 33342.
Pacific Blue, Texas Red, and Alexa Fluor are trademarks or registered trademarks of Thermo Fisher Scientific; BD Horizon is a trademark of BD Biosciences; Cy Dye is a registered trademark of Cytiva; IRDye is a registered trademark of LI-COR Biosciences.
CF® Dyes
Mix-n-Stain™ Small Ligand Labeling Kits
Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits
Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.
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Figure 2. Multiplex imaging in live HeLa cells using Mix-n-Stain™-labeled ligands. CF®488A SNAP-tag® ligand was used to detect surface expression of SNAP-ADRβ2 protein (green). Mitochondrial CF®540 CLIP-tag™ ligand was used to detect mitochondrial CLIP-Cox8A protein (red). Nuclei were stained blue with Hoechst 33342.
Figure 3. CLIP-tag™ ligand was labeled with the CF®488A Mix-n-Stain™ Small Ligand Labeling Kit, then used to detect cell surface expression of CLIP-NK1R protein (green). Cells were fixed, permeabilized, stained with CF®633 phalloidin (red), and mounted with EverBrite™ Mounting Medium with DAPI.
Figure 1. Live cell imaging of HeLa cells expressing CLIP-NK1R labeled with (A) CLIP-tag™ ligand conjugated to green fluorescent CF®488A using Mix-n-Stain™ compared to (B) CLIP-surface 488 from New England BioLabs. Cell nuclei were stained blue with Hoechst 33342.View Spectra Viewer
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Product Description
Mix-n-Stain™ Small Ligand Labeling Kits are designed for rapid labeling of low molecular weight (MW ~150 - 5000), high affinity biological ligands or substrates.
- For SNAP-tag®, CLIP-tag™ and HaloTag®, or other ligands with an aliphatic amine
- Label 100 nmol ligand
- Reaction takes ~30 minutes with minimal hands-on time and no purification
- Choose from 15 bright & photostable CF® Dyes
- Cell membrane-permeant or impermeant options for intracellular or surface targets
For more information, please download the Mix-n-Stain™ Small Ligand Labeling Kit Flyer and the Product Information Sheet. We also offer TMP-PEG3-Amine for making fluorescent TMP ligands. Also see our Mix-n-Stain™ Antibody Labeling Kits and other Reactive Dyes and Biotin Reagents.
Mix-n-Stain™ Small Ligand Labeling Kits
| Dye | Catalog no. | Spectrally similar dyes | Ex/Em (nm) | Staining |
|---|---|---|---|---|
| CF®405M | 92362 | Pacific Blue™, BD Horizon™ V450 | 408/452 | Surface |
| CF®408 | 92356 | Pacific Blue™, BD Horizon™ V450 | 408/450 | Intracellular |
| CF®488A | 92350 | FITC, Alexa Fluor® 488 | 490/515 | Surface |
| CF®500 | 92357 | FITC, Alexa Fluor® 488 | 500/510 | Intracellular |
| CF®540 | 92358 | TAMRA, TRITC, Alexa Fluor® 546, Cy®3 | 540/570 | Intracellular |
| CF®555 | 92364 | Alexa Fluor® 555, Cy®3, TRITC | 555/585 | Intracellular |
| CF®568 | 92351 | Rhodamine Red, Alexa Fluor® 568 | 562/583 | Surface |
| CF®594 | 92352 | Texas Red®, Alexa Fluor® 594 | 593/614 | Surface |
| CF®633 | 92353 | Cy®5, Alexa Fluor® 633 | 630/650 | Surface |
| CF®640R | 92354 | Cy®5, Alexa Fluor® 647 | 642/662 | Surface |
| CF®647 | 92359 | Cy®5, Alexa Fluor® 647 | 650/665 | Surface |
| CF®650 | 92363 | Cy®5, Alexa Fluor® 647 | 650/670 | Intracellular |
| CF®660C | 92360 | Alexa Fluor® 660 | 667/685 | Surface |
| CF®680 | 92361 | Cy®5.5, Alexa Fluor® 660, IRDye®680LT | 681/698 | Surface |
| CF®680R | 92355 | Cy®5.5, Alexa Fluor® 660, IRDye®680LT | 680/701 | Surface |
Antibody & Protein Labeling Kits
| Product Name | Label Options | Labeling Scale | Labeling Time | Features |
|---|---|---|---|---|
| Mix-n-Stain™ CF® Dye Antibody Labeling Kits | CF® Dyes | ≤5-20 ug IgG 20-50 ug IgG 50-100 ug IgG | ~ 15 min. | • Rapid, simple labeling • No purification • Tolerates BSA & other additives • Dyes for super-resolution, spectral flow, & NIR detection |
| Mix-n-Stain™ FITC Antibody Labeling Kits | FITC | |||
| Mix-n-Stain™ Cyanine Dye Antibody Labeling Kits | Cyanine 555 Cyanine 647 |
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| Mix-n-Stain™ Biotin Antibody Labeling Kits | Biotin | |||
| Mix-n-Stain™ Digoxigenin Antibody Labeling Kits | Digoxygenin (DIG) | |||
| Mix-n-Stain™ DNP Antibody Labeling Kits | DNP | |||
| Mix-n-Stain™ Maxi Antibody Labeling Kits | CF® Dyes Cyanine Dyes | 1 mg IgG | ~ 30 min. | |
| Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits | CF® Dyes FITC | 25 ug IgM 100 ug IgM | 15-30 min. | • Rapid, simple labeling • No purification |
| Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits | CF® Dyes | 50 ug IgG | ~ 30 min. | • Optimized to provide low 1-2.5 DOL • No purification |
| Mix-n-Stain™ Nanobody Labeling Kits | CF® Dyes | 5-20 ug Nanobody® 20-50 ug Nanobody® | ~ 30 min. | • Optimized for Nanobodies® • Tolerates BSA & other additives |
| Mix-n-Stain™ Nanobody Thiol Labeling Kits | CF® Dyes | ~ 2 hours | • Optimized for Nanobodies® with single exposed cysteine residue | |
| Mix-n-Stain™ Small Ligand Labeling Kits | CF® Dyes | 0.1 umol small ligand | ~ 30 min. | • Label SNAP®, CLIP™, HaloTag® & other small ligands • Dye options for surface or intracellular targets |
| VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging | Near-IR CF® Dyes | 3 x 1 mg IgG | ~ 2 hours | • Dyes, buffers, & spin vials for labeling + purification • Sterile syringes, filters and storage vials for in vivo use |
| CF® Dye & Biotin SE Protein Labeling Kits | CF® Dyes Biotin | 3 x 1 mg protein | ~ 2 hours | • Dyes, buffers, & spin vials for labeling + purification |
SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation.
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1 labeling
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