mouse Anti-CD2 (CD2 Antigen, LFA3 Receptor, Lymphocyte Function Antigen 2, LFA2, p50, Rosette Receptor, Sheep Erythrocyte Receptor, Sheep Red Blood Cell Receptor, SRBC, T Cell Surface Antigen CD2, T Cell Surface Antigen T11/Leu-5, T11) (FITC) Monoclonal Antibody [Clone:CC42]

Cat# C2253-02B2-100T

Size : 100Tests

Brand : US Biological



C2253-02B2 CD2 (CD2 Antigen, LFA3 Receptor, Lymphocyte Function Antigen 2, LFA2, p50, Rosette Receptor, Sheep Erythrocyte Receptor, Sheep Red Blood Cell Receptor, SRBC, T Cell Surface Antigen CD2, T Cell Surface Antigen T11/Leu-5, T11) (FITC)

Clone Type
Monoclonal
Host
mouse
Source
bovine
Isotype
IgG1
Grade
Affinity Purified
Applications
FC
Crossreactivity
Bo Gt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat; 1:10, 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: CC42|Host: mouse|Source: bovine|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Specificity: Recognizes bovine CD2. Species crossreactivity: goat.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes bovine CD2. Species crossreactivity: goat.
References
1. Davis, W.C. and Splitter, G.S. (1991) Individual antigens of cattle. Bovine CD2 (BoCD2). Vet. Immunol. Immunopathol. 27: 43-50, 2. Sopp, P et al. (1991) Investigating monoclonal antibodies to bovine "null" cell antigens using two-colour immunofluorescence. Vet. Immunol. Immunopathol. 27: 163-168, 3. Eskra, L et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets. Vet. Immunol. Immunopathol. 27: 215-229, 4. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334.