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> Mouse Anti-Filaggrin Monoclonal Antibody

Mouse Anti-Filaggrin Monoclonal Antibody

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Cat# AH13218-U0020

Size : 20ug

Brand : Abcepta


Anti-Filaggrin Antibody

Mouse Monoclonal Antibody

 
  • IHC-P - Anti-Filaggrin Antibody AH13218-20

    Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Monoclonal Antibody (FLG/1561).

Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Monoclonal Antibody (FLG/1561).

Product Information

Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, FC
Primary Accession P20930
Other Accession 654510
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse / IgG1
Clone Names FLG/1561
Calculated MW 435170 Da

Additional Information

Gene ID 2312
Other Names ATOD2; Epidermal Filaggrin; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin
Application Note Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Format 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
StorageStore at 2 to 8°C.Antibody is stable for 24 months.
PrecautionsAnti-Filaggrin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information

Name FLG
Function Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
Cellular Location Cytoplasmic granule. Note=In the stratum granulosum of the epidermis, localized within keratohyalin granules (PubMed:1429717). In granular keratinocytes and in lower corneocytes, colocalizes with calpain-1/CAPN1 (PubMed:21531719).
Tissue Location Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal gland (PubMed:19384417). In the skin, mainly expressed in stratum granulosum of the epidermis (PubMed:1429717, PubMed:19384417)
Research Areas
  • Primary Antibodies
  • Developmental Biology
  • Immunology
  • Neuroscience
  • Signal Transduction

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