Mouse IgG1 Secondary Antibody Fluorescein Conjugated
Cat# orb347540-1mg
Size : 1mg
Brand : Biorbyt
Description
Images & Validation
−
| Tested Applications | FLISA, IF, WB |
|---|---|
| Dilution Range | FLISA: 1:10,000 - 1:50,000, IF: 1:500 - 1:2,500 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Mouse IgG1 heavy chain |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA. |
| Conjugation | FITC |
Storage & Handling
−| Storage | Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody fluorescein Conjugation, Rabbit Anti-Mouse IgG1 FITC Conjugated Antibody
Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence