Quantibody® Mouse Interleukin Array 1 Kit. Detects 20 Mouse Interleukins. Suitable for all liquid sample types.
Mouse Interleukin Array Q1
Mouse Interleukin Array Q1
Contact Us for Bulk Request
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 20 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| GCSF
| GM-CSF
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
| IL-10
| IL-12 p70
| IL-13
| IL-15
| IL-17A
|
| IL-2
| IL-21
| IL-23
| IL-3
| IL-4
|
| IL-5
| IL-6
| IL-7
| IL-9
| TNF alpha
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Interleukin Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAM-INT-1.gal)
Excel (QAM-INT-1_Analysis_Tool.xls)
citations
A Membrane-Targeted Photosensitizer Prevents Drug Resistance and Induces Immune Response in Treating Candidiasis
- Species:
- Mouse
- Sample Type:
- tongues
- Year:
- 2023
- Journal Name:
- Advanced Science
Arctigenin protects against depression by inhibiting microglial activation and neuroinflammation via HMGB1/TLR4/NF‐κB and TNF‐α/TNFR1/NF‐κB pathways
- Species:
- Mouse
- Year:
- 2020
Butyrate suppresses atherosclerotic inflammation by regulating macrophages and polarization via GPR43/HDAC-miRNAs axis in ApoE−/− mice
- Species:
- Mouse
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Plos One
Fasciola hepatica fatty acid binding protein inhibits TLR4 activation and suppresses the inflammatory cytokines induced by lipopolysaccharide in vitro and in vivo
- Species:
- Mouse
- Sample Type:
- Serum (Septic Shock evaluation following LPS injection)
- Year:
- 2015
- Journal Name:
- The Journal of Immunology
- PubMed ID:
- 25780044
Immunomodulatory Response Triggered by the Alkaloids, 3-Amino- 7-Benzylbenzimidazo[3,2-a] Quinolinium Chloride (ABQ-48) and 3-Nitro- 7-Benzylbenzimidazo[3,2-a] Quinolinium Chloride (NBQ-48)
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Splenocytes treated with the various agents in title)
- Year:
- 2013
- Journal Name:
- Journal of Cancer Research and Therapeutic Oncology
- PubMed ID:
- 26086027