OGDH ELISA Kit (Human)
Cat# OKEH01369
Size : 96Wells
Brand : Aviva Systems Biology
OGDH ELISA Kit (Human) (OKEH01369)
| Datasheets/Manuals | Printable datasheet for OGDH ELISA Kit (Human) (OKEH01369) |
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| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology OGDH ELISA Kit (Human) (OKEH01369) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OGDH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OGDH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OGDH captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 3.8% (n = 20) Mean Inter-assay CV%: < 7.2% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.01 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human 2-oxoglutarate dehydrogenase, mitochondrial | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | OGDH |
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| Gene Full Name | oxoglutarate dehydrogenase |
| Alias Symbols | 2-oxoglutarate dehydrogenase complex component E1, 2-oxoglutarate dehydrogenase, mitochondrial, AKGDH, Alpha-ketoglutarate dehydrogenase, E1k, KGD1, OGDC, OGDC-E1, OGDH2, oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide), oxoglutarate decarboxylase, oxoglutarate dehydrogenase (succinyl-transferring), testicular tissue protein Li 131. |
| NCBI Gene Id | 4967 |
| Protein Name | 2-oxoglutarate dehydrogenase, mitochondrial |
| Description of Target | 2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex, which mediates the decarboxylation of alpha-ketoglutarate (PubMed:24495017). The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2) (PubMed:24495017). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (PubMed:29211711). A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed:29211711). |
| Uniprot ID | Q02218 |
| Protein Accession # | NP_001003941.1 |
| Nucleotide Accession # | NM_001003941.2 |
| Protein Size (# AA) | 1023 |
| Molecular Weight | 116 kDa |
