OREXIN A AND B ELISA Kit (Sheep)
Cat# OKWB00437
Size : 96Wells
Brand : Aviva Systems Biology
OREXIN A AND B ELISA Kit (Sheep) (OKWB00437)
| Datasheets/Manuals | Printable datasheet for OREXIN A AND B ELISA Kit (Sheep) (OKWB00437) |
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| Predicted Species Reactivity | Ovis aries|Sheep | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | Aviva Systems Biology Orexin a and b ELISA Kit (Sheep) (OKWB00437) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Orexin a and b has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Orexin a and b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Orexin a and b captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 15.625-1000ng/ml | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Mean Intra-Assay Precision <10% Mean Inter-Assay Precision <12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 9.4 pg/mL | ||||||||||||||||||||||
| Assay Info | Assay Type: Quantitative Sandwich ELISA |
| Gene Symbol | OREXIN A AND B |
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| Protein Name | Orexin A and B |
| Uniprot ID | Q95L34 |