Pyruvate Kinase (PK) Colorimetric Assay Kit
Cat# MBS9719073-96
Size : 96Tests
Brand : MyBiosource
Pyruvate Kinase (PK) Assay Kit
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9719073PROMOTIONAL PRICING: 5% OFF web . Discount is not applicable for bulk pricing such as 5x96T or 10x96T. Discount will be applied after order has been placed
Principle of the Assay: Pyruvate Kinase (PK, EC 2.7.1.40) is widely present in animals, plants, microorganisms and cultured cells, and catalyzes the last step reaction in the glycolysis process. It is one of the main rate-limiting enzymes in the glycolysis… More details
Product Overview
Online Datasheet in PDFProduct Name
Pyruvate Kinase (PK), Assay Kit
Full Product Name
Pyruvate Kinase (PK) Colorimetric Assay Kit
Product Gene Name
PK assay kit
Samples
Serum, Plasma, Animal and Plant Tissues, Cells, Bacteria
Preparation and Storage
Storage at -20 degree C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping: Gel pack with blue ice.
Shipping: Gel pack with blue ice.
Features & Benefits
Determination of Pyruvate kinase (PK) activity in cells, bacteria, serum (plasma), tissue/cell lysates and other biological fluids.
Kit components and procedures are simple.
Sample preparation, experimental steps and result calculation are more detailed and accurate.
Long shelf life.
Kit components and procedures are simple.
Sample preparation, experimental steps and result calculation are more detailed and accurate.
Long shelf life.
Usage Notes
Assay kit is intended for research use only. Not for use in test procedures. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Do not mix or substitute kit reagents or materials from other lots or vendors. Kits are QC tested as a set of components and performance cannot be guaranteed if utilized separately or substituted.
Avoid foaming or bubbles when mixing or reconstituting components.
Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
Ensure all reagents and equipment are at the appropriate temperature before assay.
96-well UV microplate is needed
Sample preparation and other processes need to be performed on ice, and the enzyme activity must be measured on the same day and avoid repeated freezing and thawing to prevent enzyme inactivation.
LDH must be placed on ice during the measurement process. The reaction temperature has an influence on the results, so please keep it at 25 degree C(general species) or 37 degree C(mammal).
Do not mix or substitute kit reagents or materials from other lots or vendors. Kits are QC tested as a set of components and performance cannot be guaranteed if utilized separately or substituted.
Avoid foaming or bubbles when mixing or reconstituting components.
Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
Ensure all reagents and equipment are at the appropriate temperature before assay.
96-well UV microplate is needed
Sample preparation and other processes need to be performed on ice, and the enzyme activity must be measured on the same day and avoid repeated freezing and thawing to prevent enzyme inactivation.
LDH must be placed on ice during the measurement process. The reaction temperature has an influence on the results, so please keep it at 25 degree C(general species) or 37 degree C(mammal).
Product Categories/Family for PK assay kit
Cell Metabolism
Other Notes
Small volumes of PK assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PK purchase
MBS9719073 is an assay kit targeting PK. Also known as Pyruvate Kinase (PK) Colorimetric Assay Kit.
Additional Details
NCBI/Uniprot data below describe general gene information for PK. It may not necessarily be applicable to this product.
Protein Family
Pyridoxal kinase
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.