Reagents and instruments for immunology, cell biology and molecular biology.

Rabbit Anti-CALB1 Polyclonal Antibody

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Cat# HPA023099

Size : 100ul

Brand : Atlas Antibodies

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HPA023099

calbindin 1, 28kDa

Anti-CALB1 Antibody

Polyclonal Antibody against HUMAN CALB1

0.3 mg/ml

info@clinisciences.com

Immunohistochemistry analysis in human kidney and skeletal muscle tissues using HPA023099 antibody. Corresponding CALB1 RNA-seq data are presented for the same tissues.

Product details

Target protein: calbindin 1, 28kDa
Target gene: CALB1
Verified species reactivity: Human
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000028222 (98%)RAT - ENSRNOG00000007456 (99%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

Unit size: 100µl
Current lot: A104741
Concentration: 0.3 mg/ml

Applications

Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
- Working concentration: 0.25-2 µg/ml
- Fixation/Permeabilization: PFA/Triton X-100
Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:5000 - 1:10000
- Retrieval method: HIER pH6
Western Blot (WB) recommended conditions
- Working concentration: 0.04-0.4 µg/ml

Validation

Immunofluorescence in Cell Lines (ICC-IF)
Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. Validation of protein expression in IHC by comparing independent antibodies targeting different epitopes of the protein. Validated against independent antibody
Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above)

Target information

Protein name: calbindin 1, 28kDa
Gene name: CALB1
Alternative gene names: CALB,
Ensembl:
Entrez:
UniProt:

Shipping and storage

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (4)

Tao Y, Vermilyea SC, Zammit M, Lu J, Olsen M, Metzger JM, Yao L, Chen Y, Phillips S, Holden JE, Bondarenko V, Block WF, Barnhart TE, Schultz-Darken N, Brunner K, Simmons H, Christian BT, Emborg ME, Zhang SCNat Med , 2021 Mar 1; 27(4):632-639. Epub 2021 Mar 12021 Mar 1
Liang CY, Li ZY, Gan TQ, Fang YY, Gan BL, Chen WJ, Dang YW, Shi K, Feng ZB, Chen GRespir Res , 2020 Feb 26; 21:60. Epub 2020 Feb 262020 Feb 26