Rabbit Anti-CARD11 Polyclonal Antibody

Western blot analysis of CARD11 expression in K562 (A), Ramos (B), Jurkat (C) whole cell lysates. (Predicted band size: 133 kD; Observed band size: 133 kD)

Immunofluorescent analysis of CARD11 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).