Apolipoprotein E (ApoE) produced by the liver and circulating macrophages, that is a constituent of every plasma lipoprotein except the smallest low density lipoproteins (LDL). It is a key player in the recycling and redistribution of lipids and cholesterol. ApoE is a ligand with a high affinity for low density lipoprotein receptors (LDLR). It regulates the activity of enzymes that metabolize lipids and also makes lipids soluble. Mice and humans that lack ApoE cannot remove excess lipoproteins from the plasma and have an increased risk of atherosclerosis. Defective binding of ApoE to its receptors will lead to accumulation of cholesterolrich lipoprotein particles in the plasma; this is the cause of type III hyperlipoproteinemia. There are three main isoforms of ApoE all product of alleles at a single gene locus: E2 (Cys112, Cys158), E3 (C112, Arg158), and E4 (Arg112, Arg158). Inheritance of ApoE2 has been associated with lower levels of total cholesterol, lowdensity lipoprotein cholesterol and nonhighdensity lipoprotein cholesterol.

E2 is associated with the genetic disorder type III hyperlipoproteinemia and with both increased and decreased risk for atherosclerosis.

Data not yet available.

MKVEQAVETE PEPELRQQTE WQSGQRWELA LGRFWDYLRW VQTLSEQVQE ELLSSQVTQE LRALMDETMK ELKAYKSELE EQLTPVAEET RARLSKELQA AQARLGADME DVCGRLVQYR GEVQAMLGQS TEELRVRLAS HLRKLRKRLL RDADDLQKCL AVYQAGAREG AERGLSAIRE RLGPLVEQGR VRAATVGSLA GQPLQERAQA WGERLRARME EMGSRTRDRL DEDEVKEQVAEV RAKLEEQAQQ IRLQAEAFQA RLKSWFEPLV EDMQRQWAGL VEKVQAAVGT SAAPVPSDNH

Lyophilized

The molecular weight of Recombinant Human ApoE2 is Mr 34.4 kDa.

This product is 0.2 micron sterile filtered and lyophilized from 20 mM Sodium Phosphate, pH 7.8 + 0.5mM DTT.

The lyophilized protein should be stored desiccated at 20°C. The reconstituted protein can be stored for at least one week at 28°C. For longterm storage of the reconstituted protein, aliquot into working volumes and store at 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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