Recombinant Human IFN gamma
Cat# CI57-1mg
Size : 1mg
Brand : Novoprotein
Description Recombinant Human Interferon Gamma is produced by our Mammalian expression system and the target gene encoding Gln24-Gln166 is expressed.
Accession P01579
Known As Interferon Gamma; IFN-Gamma; Immune Interferon; IFNG
Mol Mass 16.8 KDa
APMol Mass 16&20&25 KDa, reducing conditions
Formulation Description Lyophilized from a 0.2 μm filtered solution of 20mM PB, 4% Mannitol, 2% Sucrose, 0.02% Tween80, pH7.4.
Endotoxin < 0.01 EU/µg as determined by LAL test.
Purity-SDS-PAGE 
Greater than 95% as determined by reducing SDS-PAGE. (QC verified) Purity-SEC-HPLC 
Greater than 95% as determined by SEC-HPLC. (Regularly tested) Bioactivity-Cell Based Assay 
Measured by its ability to inhibit the proliferation of HT-29 cells. The ED50 for this effect is 0.15-0.75 ng/mL. Reconstitution - Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
- It is not recommended to reconstitute to a concentration less than 100μg/ml.
- Dissolve the lyophilized protein in distilled water.
- Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage - Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping - The product is shipped at ambient temperature.
- Upon receipt, store it immediately at the temperature listed below.
Background IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF.