Recombinant Rat Flap endonuclease 1 (Fen1)
Cat# MBS1443644-0,1mg-M
Size : 0.1mg(Mammalian-Cell)
Brand : MyBiosource
Flap Endonuclease 1 (FEN1) Rat Recombinant Protein
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1443644This protein removes 5 overhanging flaps in DNA repair and processes the 5 ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides… More details
Product Overview
Online Datasheet in PDFProduct Name
Flap endonuclease 1 (Fen1), Recombinant Protein
Full Product Name
Recombinant Rat Flap endonuclease 1 (Fen1)
Product Gene Name
Fen1 recombinant protein
Product Synonym Gene Name
Fen1
Sequence
MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYIFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQEAGAE EEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALAKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RVLQELGLNQ EQFVDLCILL GSDYCESVRG IGPKRAVDLI QKHKSIEEIV RRLDPSKYPV PENWLHKEAR QLFLEPEVLD PESVELKWSE PNEEELVKFM CGEKQFSEER IRSGVKRLNK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGPAKKKAKT GGAGKFRRGK
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
42,609 Da
Sequence Positions
1-380, full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Rat
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of Fen1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Fen1 purchase
MBS1443644 is a recombinant protein targeting Fen1. Also known as Recombinant Rat Flap endonuclease 1 (Fen1). Host: E Coli or Yeast or Baculovirus or Mammalian Cell.
Additional Details
NCBI/Uniprot data below describe general gene information for Fen1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
flap endonuclease 1
NCBI Official Synonym Full Names
flap structure-specific endonuclease 1
NCBI Official Symbol
Fen1
NCBI Official Synonym Symbols
FEN-1
UniProt Protein Name
Flap endonuclease 1
UniProt Synonym Protein Names
Flap structure-specific endonuclease 1
UniProt Gene Name
Fen1
UniProt Synonym Gene Names
FEN-1
NCBI Protein Information
flap endonuclease 1
NCBI Summary for Fen1
removes RNA primers during Okazaki fragment maturation in DNA synthesis [RGD, Feb 2006]
UniProt Comments for Fen1
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Show more Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with Fen1 recombinant protein
| Reference Product | PubMed Publications |
|---|---|
| Pcna recombinant protein | >107 publications with Fen1 and Pcna |
| Apex1 recombinant protein | >18 publications with Fen1 and Apex1 |
| Rpa1 recombinant protein | >9 publications with Fen1 and Rpa1 |
| Pold3 recombinant protein | >2 publications with Fen1 and Pold3 |
| Pola1 recombinant protein | >2 publications with Fen1 and Pola1 |
Pathways associated with Fen1 recombinant protein
| Products by Pathway | Pathway Diagram |
|---|---|
| Base Excision Repair Pathway antibodies | Base Excision Repair Pathway Diagram |
| Base Excision Repair Pathway antibodies | Base Excision Repair Pathway Diagram |
| Base Excision Repair Pathway antibodies | Base Excision Repair Pathway Diagram |
| Cell Cycle Pathway antibodies | Cell Cycle Pathway Diagram |
| Cell Cycle, Mitotic Pathway antibodies | Cell Cycle, Mitotic Pathway Diagram |
| Chromosome Maintenance Pathway antibodies | Chromosome Maintenance Pathway Diagram |
| DNA Repair Pathway antibodies | DNA Repair Pathway Diagram |
| DNA Replication Pathway antibodies | DNA Replication Pathway Diagram |
| DNA Replication Pathway antibodies | DNA Replication Pathway Diagram |
| DNA Replication Pathway antibodies | DNA Replication Pathway Diagram |
Diseases associated with Fen1 recombinant protein
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >36 publications with Fen1 and Neoplasms |
| Carcinoma Antibodies | >13 publications with Fen1 and Carcinoma |
| Lung Neoplasms Antibodies | >9 publications with Fen1 and Lung Neoplasms |
| Skin Diseases Antibodies | >8 publications with Fen1 and Skin Diseases |
| Drug Toxicity Antibodies | >8 publications with Fen1 and Drug Toxicity |
| Nervous System Diseases Antibodies | >8 publications with Fen1 and Nervous System Diseases |
| Adenocarcinoma Antibodies | >8 publications with Fen1 and Adenocarcinoma |
| Brain Diseases Antibodies | >7 publications with Fen1 and Brain Diseases |
| Breast Neoplasms Antibodies | >6 publications with Fen1 and Breast Neoplasms |
| Chromosome Aberrations Antibodies | >6 publications with Fen1 and Chromosome Aberrations |
Organs/Tissues associated with Fen1 recombinant protein
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Lung Antibodies | >11 publications with Fen1 and Lung |
| Testis Antibodies | >7 publications with Fen1 and Testis |
| Brain Antibodies | >7 publications with Fen1 and Brain |
| Colon Antibodies | >6 publications with Fen1 and Colon |
| Prostate Antibodies | >4 publications with Fen1 and Prostate |
| Spleen Antibodies | >2 publications with Fen1 and Spleen |
| Connective Tissue Antibodies | >2 publications with Fen1 and Connective Tissue |
| Ovary Antibodies | >1 publications with Fen1 and Ovary |
| Kidney Antibodies | >1 publications with Fen1 and Kidney |
| Uncharacterized Tissue Antibodies | >1 publications with Fen1 and Uncharacterized Tissue |