RH IL-21 (AF)
Cat# 100-90AF-10UG
Size : 10UG
Brand : FUJIFILM
Recombinant Human IL-21
Interleukin 21 (IL-21) is a member of the common-gamma chain family of cytokines that are involved in immunoregulation. IL-21 is normally expressed by activated CD4+ T cells and is aberrantly expressed in Hodgkin lymphoma cells. The IL-21 receptor (IL-21R) activates the JAK/STAT signaling pathway and is expressed on T, B, and natural killer (NK) cells. Within the B cell lineage, IL-21 is a switch factor regulating IgG1 and IgG3 antibody production. IL-21 also cooperates with IL-4 for the production of multiple antibody classes in B cells. IL-21 has pleiotropic effects on the proliferation, differentiation, and effector functions of B, T, NK, and dendritic cells.
Also available as a GMP product: CTGrade GMP rh IL-21
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
Cell based proliferation assay
Figure 1. Recombinant Human IL-21 induced proliferation of B9 Cells
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | None |
| Accession Number | Q9HBE4 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 15.4 kDa (132 aa) |
| AA Sequence | MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 97% |
| Endotoxin | Kinetic LAL | ≤ 1 EU/µg |
| Biological Activity (ED50) | B9 cell proliferation | ≤ 5 ng/mL; ≥ 2.0 x 105 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.