Reagents and instruments for immunology, cell biology and molecular biology.

Spathulenol [6750-60-3]

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Cat# HY-N1205-1mg

Size : 1mg

Brand : MedChemExpress

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Description

Spathulenol is isolated from Aristolochia yunnanensis, and has antioxidant, anti-inflammatory, antiproliferative and antimycobacterial activities. Spathulenol shows a high antioxidant activity with an IC₅₀ of 85.60 μg/mL in the DPPH system^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	ED₅₀	>20 μg/mL Compound: (+)-Spathulenol	Cytotoxicity against human A431 cells Cytotoxicity against human A431 cells	[PMID: 1517737]
BC1 cell line	ED₅₀	>20 μg/mL Compound: (+)-Spathulenol	Cytotoxicity against human BC1 cells Cytotoxicity against human BC1 cells	[PMID: 1517737]
BC1 cell line	ED₅₀	>20 μg/mL Compound: spathulenol	Cytotoxicity against human BC1 cells Cytotoxicity against human BC1 cells	[PMID: 7931367]
Col2	ED₅₀	>20 μg/mL Compound: (+)-Spathulenol	Cytotoxicity against human Col2 cells Cytotoxicity against human Col2 cells	[PMID: 1517737]
Col2	ED₅₀	>20 μg/mL Compound: spathulenol	Cytotoxicity against human Col2 cells Cytotoxicity against human Col2 cells	[PMID: 7931367]
HT-1080	ED₅₀	>20 μg/mL Compound: (+)-Spathulenol	Cytotoxicity against human HT1080 cells Cytotoxicity against human HT1080 cells	[PMID: 1517737]
HT-1080	ED₅₀	>20 μg/mL Compound: spathulenol	Cytotoxicity against human HT1080 cells Cytotoxicity against human HT1080 cells	[PMID: 7931367]
KB	ED₅₀	>20 μg/mL Compound: (+)-Spathulenol	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 1517737]
KB	ED₅₀	>20 μg/mL Compound: spathulenol	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 7931367]
KB-V1	ED₅₀	>20 μg/mL Compound: (+)-Spathulenol	Cytotoxicity against human vinblastine-resistant KBV1 cells Cytotoxicity against human vinblastine-resistant KBV1 cells	[PMID: 1517737]
KB-V1	ED₅₀	>20 μg/mL Compound: spathulenol	Cytotoxicity against human KBV1 cells Cytotoxicity against human KBV1 cells	[PMID: 7931367]
LNCaP	ED₅₀	>20 μg/mL Compound: (+)-Spathulenol	Cytotoxicity against human LNCAP cells Cytotoxicity against human LNCAP cells	[PMID: 1517737]
Lu1	ED₅₀	>20 μg/mL Compound: (+)-Spathulenol	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 1517737]
Lu1	ED₅₀	>20 μg/mL Compound: spathulenol	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 7931367]
P388	ED₅₀	>20 μg/mL Compound: (+)-Spathulenol	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 1517737]
P388	ED₅₀	>20 μg/mL Compound: spathulenol	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 7931367]
SK-MEL-2	ED₅₀	>20 μg/mL Compound: (+)-Spathulenol	Cytotoxicity against human SK-MEL-2 cells Cytotoxicity against human SK-MEL-2 cells	[PMID: 1517737]
SK-MEL-2	ED₅₀	6.3 μg/mL Compound: spathulenol	Cytotoxicity against human SK-MEL-2 cells Cytotoxicity against human SK-MEL-2 cells	[PMID: 7931367]
ZR-75-1	ED₅₀	>20 μg/mL Compound: (+)-Spathulenol	Cytotoxicity against human ZR-75-1 cells Cytotoxicity against human ZR-75-1 cells	[PMID: 1517737]

In Vitro

Spathulenol exhibits a high antioxidant activity s in the DPPH and MDA system, exhibits significant DPPH free radical activity, with IC₅₀ values of 85.60 μg/mL^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (453.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.5382 mL	22.6912 mL	45.3823 mL
5 mM	0.9076 mL	4.5382 mL	9.0765 mL
10 mM	0.4538 mL	2.2691 mL	4.5382 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Purity & Documentation

Purity: 99.20%

Data Sheet (282 KB) SDS (393 KB)

COA (262 KB) HNMR (191 KB) CNMR (191 KB) RP-HPLC (251 KB) MS (118 KB)

Handling Instructions (2659 KB)

References

[1]. Nascimento KF, et al. Antioxidant, anti-inflammatory, antiproliferative and antimycobacterial activities of the essential oil of Psidium guineense Sw. and spathulenol.J Ethnopharmacol. 2018 Jan 10;210:351-358. [Content Brief]

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Spathulenol [6750-60-3]

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Brand : MedChemExpress

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