Terminal deoxyribonucleotidyltransferase [9027-67-2]

Terminal deoxyribonucleotidyltransferase (TdT) catalyses the condensation of deoxyribonucleotide triphosphates onto the 3' hydroxyl ends of DNA strands and adds N-regions to gene segment junctions during V(D)J recombination. Terminal deoxyribonucleotidyltransferase is expressed in immature, pre-B, pre-T lymphoid cells, and acute lymphoblastic leukemia/lymphoma cells^[1].

9027-67-2

Liquid

Colorless to light yellow

[Terminal deoxyribonucleotidyltransferase]

5 U/μL

One unit is defined as the amount of enzyme that can catalyse the addition of 1 nmol of dNTP to the 3'hydroxyl end of the polynucleotide within 60 min at 37°C.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Boulé JB, et, al. Terminal deoxynucleotidyl transferase indiscriminately incorporates ribonucleotides and deoxyribonucleotides. J Biol Chem. 2001 Aug 17;276(33):31388-93.  [Content Brief]