Thiazole Green, is structurally identical to the well known qPCR and DNA gel stain SYBR® Green I. Thiazole Green is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR. Thiazole Green can also be used to detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any prewashing steps, although the sensitivity is lower. Thiazole Green may also be used as a green nuclear stain for all cells in live cultures, but will lose nuclear specificity after fixation. Thiazole Green has the identical spectral properties as SYBR® Green I (Ex/Em 498/522 nm with DNA).