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TIE2 Protein, Human, Recombinant (His)

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Cat# NB-64-56234-10ug

Size : 10ug

Brand : Neo Biotech

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TIE2 Protein, Human, Recombinant (His)

(Synonyms: VMCM1, VMCM, TIE-2, TIE2, TEK tyrosine kinase, endothelial, CD202B) Copy Product Info

Synonyms: VMCM1, VMCM, TIE-2, TIE2, TEK tyrosine kinase, endothelial, CD202B

Catalog No. TMPY-01064 Copy Product Info

Purity: 99.4%

TIE2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 82 kDa and the accession number is AAA61139.1.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.4%

Appearance:Lyophilized powder

Color:White

Product Information

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized recombinant human Tie2 at 2 μg/ml (100 μl/well) can bind human Angiopoietin-2 at a linear range of 1.28-160 ng/ml.
Description	TIE2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 82 kDa and the accession number is AAA61139.1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	AAA61139.1
Construction	A DNA sequence encoding the extracellular domain (Met1-Lys745) of human Tie2 (AAA61139.1) precursor was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Ala 23
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	VMCM1, VMCM, TIE-2, TIE2, TEK tyrosine kinase, endothelial, CD202B
Research Background	TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Chemical Properties

Molecular Weight	82 kDa (predicted); 95-105 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

VMCM-1VMCM 1TIE 2

Related Tags: TIE2 Protein, Human, Recombinant (His) chemical structure | TIE2 Protein, Human, Recombinant (His) molecular weight