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> TIF1 gamma (TRIM33) (NM_015906) Human Over-expression Lysate

TIF1 gamma (TRIM33) (NM_015906) Human Over-expression Lysate

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Cat# LY414337

Size : 100ug


TIF1 gamma (TRIM33) (NM_015906) Human Over-expression Lysate

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SKU
LY414337
Transient overexpression lysate of tripartite motif-containing 33 (TRIM33), transcript variant a (as WB positive control)
 

Other products for "TIF1 gamma or NM_015906"

Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC212497
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_015906, NP_056990
Synonyms ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA; TIFGAMMA
Predicted MW 122.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_056990
Locus ID 51592
Cytogenetics 1p13.2
Protein Families WB
Product Manuals
  • Western Blot Protocols
FAQs
  • Over-expression Cell Lysate
SDS
SKU Description Size
PH312497 TRIM33 MS Standard C13 and N15-labeled recombinant protein (NP_056990) 10 ug
LC414337 Lyophilized TRIM33 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
TP312497 Recombinant protein of human tripartite motif-containing 33 (TRIM33), transcript variant a, 20 µg 20 ug
TP710251 Purified recombinant protein of Human tripartite motif containing 33 (TRIM33), transcript variant a, full length, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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