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Triptolide [38748-32-2]

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Cat# NB-64-11248-5mg

Size : 5mg

Brand : Neo Biotech

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Triptolide

(Synonyms: PG490, NSC 163062) Copy Product Info

Synonyms: PG490, NSC 163062

Catalog No. T2179 Copy Product Info

Purity: 99.83%

Triptolide (PG490) belongs to the tricyclic diterpenoid group of natural products and is an inhibitor of NF-κB activation. Triptolide exhibits immunosuppressive, anti-rheumatic, anti-inflammatory, anti-proliferative and anti-tumor activities.

Cas No. 38748-32-2

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.83%

Color:White

Product Introduction

Bioactivity

Description	Triptolide (PG490) belongs to the tricyclic diterpenoid group of natural products and is an inhibitor of NF-κB activation. Triptolide exhibits immunosuppressive, anti-rheumatic, anti-inflammatory, anti-proliferative and anti-tumor activities.
Targets & IC50	MDM2-p53:47-73 nM (IC50)
In vitro	METHODS: Neuroblastoma cells BE(2)-C were treated with Triptolide (5-100 nM) for 24 h. Cell viability was measured using the CCK-8 Assay. RESULTS: A concentration-dependent response to Triptolide was observed in BE(2)-C cells. At 50 nM Triptolide, cell viability was significantly reduced to 50%. [1] METHODS: Breast cancer cells MCF-7 were treated with Triptolide (10-50 nmol/L) for 24 h, and the expression levels of target proteins were detected using Western Blot. RESULTS: Triptolide decreased the messenger RNA and protein levels of ERα in MCF-7 cells in a dose-dependent manner. [2]
In vivo	METHODS: To assay antitumor activity in vivo, Triptolide (0.4 mg/kg) was administered by gavage to BALB/c-nu+/nu+ mice harboring the breast cancer tumor MCF-7 once daily for three weeks. RESULTS: Triptolide inhibited the growth of MCF-7 cell xenografts in a mouse model. [2] METHODS: To investigate the effects on ulcerative colitis (UC), Triptolide (0.1-0.4 mg/kg) was administered orally to DSS-induced UC mice once daily for seven days. RESULTS: Triptolide has anti-inflammatory and therapeutic effects on UC mice. [3]
Synonyms	PG490, NSC 163062
Cell Research	Triptolide is dissolved in DMSO (1 mg/mL) and stored, and then diluted with RPMI 1640 medium before use[3]. The viability of differentiated PC12 cells treated with different concentrations of Triptolide. After differentiated PC12 cells are cultured on 96-well plates with RPMI 1640 medium for stabilization, differentiated PC12 cells are incubated with different concentrations of Triptolide (0.01, 0.1, and 1 nM) for 24 hours. The concentrations in this study are chosen. Then cell viability is determined by the MTT assay. Each condition and experiment is repeated three times[3].

Chemical Properties

Molecular Weight	360.40
Formula	C₂₀H₂₄O₆
Cas No.	38748-32-2
Smiles	10[C@@]12C[C@@H]3O[C@@]33[C@H](O)[C@]4(O[C@H]4[C@@H]4O[C@]34[C@@]1(C)CCC1=C2COC1=O)C(C)C
Relative Density.	1.48 g/cm3

Storage & Solubility Information

Storage

Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 51.00 mg/mL (141.51 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7747 mL	13.8735 mL	27.7469 mL	138.7347 mL
5 mM	0.5549 mL	2.7747 mL	5.5494 mL	27.7469 mL
10 mM	0.2775 mL	1.3873 mL	2.7747 mL	13.8735 mL
20 mM	0.1387 mL	0.6937 mL	1.3873 mL	6.9367 mL
50 mM	0.0555 mL	0.2775 mL	0.5549 mL	2.7747 mL
100 mM	0.0277 mL	0.1387 mL	0.2775 mL	1.3873 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

Keywords

TriptolidePG-490PG 490Nuclear factor-κBNuclear factor-kappaBNSC-163062NSC163062NF-κBNFκBNF-kBNFkBMdm2Inhibitorinhibitheat shock factor (HSF1)( in ALL cell lines)Apoptosis

Related Tags: Triptolide chemical structure | Triptolide in vivo | Triptolide in vitro | Triptolide formula | Triptolide molecular weight