Vimentin antibody
Cat# GTX112661-25ul
Size : 25ul
Brand : Genetex
Host | Rabbit |
---|---|
Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA |
Reactivity | Human |
Package | 100 μl, 25 μl |
APPLICATION
Application Note
Application | Recommended Dilution |
---|---|
WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
ELISA | Assay dependent |
Sandwich ELISA | Assay dependent |
Note : Sandwich ELISACapture : GTX629744 / GTX629743, Detection : GTX112661 |
Calculated MW
Positive Control
Predict Reactivity
PROPERTIES
Form
Buffer
Preservative
Storage
Concentration
Antigen Species
Immunogen
Purification
Conjugation
RRID
Note
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Background
Database
DATA IMAGES
GTX112661 WB ImageNon-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. |
GTX112661 IHC-P Image Vimentin antibody detects VIM protein at cytosol on U373 xenograft by immunohistochemical analysis. |
GTX112661 ELISA ImageSandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody. |
GTX112661 ICC/IF ImageImmunofluorescence analysis of methanol-fixed HeLa, using Vimentin(GTX112661) antibody at 1:200 dilution. |
GTX112661 IHC-P Image Vimentin antibody detects Vimentin protein at secreted on human ovarian carcinoma by immunohistochemical analysis. |
GTX112661 IHC-P Image Vimentin antibody detects VIM protein at cytosol on HBL435 xenograft by immunohistochemical analysis. |
GTX112661 WB ImageVarious whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX112661 IHC-P Image Vimentin antibody detects Vimentin protein at secreted on human breast carcinoma by immunohistochemical analysis. |
GTX112661 WB ImageWhole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX112661 WB ImageVarious whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
GTX112661 ELISA ImageSandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody. |
GTX112661 WB ImageNon-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
Application Reference
REVIEW
SDS | |
---|---|
PBS.pdf | |
Glycerol.pdf | |
Proclin.pdf |