HRP Chromogen for IHC - DAB
DAB (3,3'-Diaminobenzidine) is the most widely used chromogen for use with HRP. DAB substrate forms a brown colored deposit when it reacts with peroxidase in the presence of hemoglobin.
The use of DAB is simple, simply incubate the slides for 5 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under the microscope.
When staining is complete, it is necessary to rinse the slides with water for 1-2 minutes to remove excess DAB. The precipitate from DAB is insoluble in alcohol and other organic solvents. Samples can be dehydrated and slides mounted permanently (O-Mount).
Name
DAB (3,3'-Diaminobenzidine)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Brown
Microscopy
Brightfield, Darkfield, Electron, Spectral Imaging
Mounting
Aqueous (Hardening), Non-Aqueous (Permanent)
Other properties
Heat Resistant, Multiple Labeling
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- Unconjugated 849
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- PE 5
- human 348
- e. coli 70
- cow 40
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- e. coli 301
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- 36
- horse 12
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- bird 5
- cat 1
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- Protein/peptide 1723
- Biochemicals 1498
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- ELISA/ELISPOT 354
- Primary antibody 307
- Buffers and reagents 179
- kit 108
- Probe 62
- Other products 22
- cDNA Clone 19
- RNAi 16
- Secondary antibody 15
- Lipids 2
- Primary cells 2
- CRISPR/Cas9 1
- Paraffin tissue section 1
- Primer 1
- ELISA 374
- IHC 96
- WB 22
- IP 8
- IF 7
- FA 6
- FC 6
- ICC 5
- Chemiluminescence 3
- Dot blot 3
- in-vivo 2
- ISH 1
- PCR 1
- Polyclonal 22
- Monoclonal 8
- 10.1F8 5
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- 13.2G8 5
- 13.6D9 5
- 13.7H4 5
- 13B4.E10 5
- 14.2H9 5
- 26.7C4 5
- 3G8 5
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- 6.1H3.D9.C6 5
- 6.1H8.F9.G6 5
- 6.7A2.A4.D3 5
- 6.8E7.A7.A1 5
- 8C7 5
- 8G1 5
- 8H6 5
- 9.21C1.G1.F1.A5 5
- 9.22E1.F5.H10 5
- 9.24E10.F3.H12 5
- 12H6.D3 4
- 13.7E2 4
- 13.8E5 4
- 14F10.H9 4
- 1A6 4
- 2E4 4
- 4B9 4
- 5A11 4
- 5A2 4
- 6.2D2.E1.D11 4
- 6.8A2.B8.A11 4
- 8H12 4
- IgG.2A5 4
- IgG.2E9 4
- 3
- 001.11D9 3
- 001.11F2 3
- 001.6C2 3
- 001.7B11 3
- E1A 3
- E1B 3
- Melredableukin Alfa 2
- 13.7E2. 1
- 13.8E5. 1
- 6. 8A2.B8.A11 1
- Recombinant 1
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