Size exclusion resins (SEC)
Size exclusion chromatography separates proteins by their hydrodynamic radius, a property determined both by the size and shape of the molecule. Unlike other chromatographic methods, proteins do not bind to a stationary phase during size exclusion chromatography (SEC). In contrast, proteins are separated by the rate at which they navigate through an inert stationary phase called size exclusion resin.
SEC is a method most often used in the final stages of a purification system because of its ability to differentiate between different species of a protein. Oligomeric species, unfolded proteins, and truncated proteins can all be separated from the native protein, while simultaneously exchanging components of the buffer. Often, size exclusion chromatography is used as a faster and more reliable buffer exchange method than dialysis because it is compatible with several solvents and requires less buffer. A single solvent is used throughout the procedure, and the available resins are compatible with the most commonly used buffers.
The type of resin used and the length of the column play a critical role in solving SEC-separated proteins. The choice of the resin depends on the molecular weight of the proteins that require separation and the conditions under which the separation will be performed.
Frequently Asked Questions (FAQ)
Where can I find size exclusion (sec) products on the CliniSciences website?
size exclusion (sec) products are available in this section and can be sorted by product families or sub-categories, such as 6-5000 kDa, 6-600 kDa, 3-70 kDa. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the size exclusion (sec) category?
The size exclusion (sec) category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The 6-600 kDa, 3-70 kDa, 6-5000 kDa sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a size exclusion (sec) product?
To find a size exclusion (sec) product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does CliniSciences stock several product codes in the size exclusion (sec) category?
Yes, CliniSciences distributes several product references in the size exclusion (sec) category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for size exclusion (sec) products?
Yes, you can request a quote for size exclusion (sec) products from the product page or by contacting CliniSciences (info@clinisciences.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a size exclusion (sec) product is available to order?
The availability of a size exclusion (sec) product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact CliniSciences with the product code or name of the product you are looking for.
What should I do if I cannot find the size exclusion (sec) product I am looking for?
If the size exclusion (sec) product you are looking for does not appear in the catalogue, please contact CliniSciences with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all size exclusion (sec) products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.