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> Neuroligin 3 Antibody

Neuroligin 3 Antibody

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Cat# ASM10303-A565-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - Neuroligin 3 Antibody ASM10303
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29 (ASM10303). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody (ASM10303) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction, Synapse . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Neuroligin 3 Antibody (D) Composite.
  • WB - Neuroligin 3 Antibody ASM10303
    Western Blot analysis of Mouse Brain Membrane showing detection of ~110 kDa Neuroligin 3 protein using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone S110-29 (ASM10303). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody (ASM10303) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF
Primary Accession Q62889
Other Accession AAA97871
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat Neuroligin 3 Monoclonal IgG1
Target/Specificity Detects ~110kDa. Does not cross-react with Neuroligin-1, -2, -4 or -4.
Other Names Gliotactin homolog Antibody, Neuroligin-3 Antibody, Nlgn3 Antibody, NLGN3_HUMAN Antibody
Clone Names S110-29
Immunogen Fusion protein amino acids 730-848 (intracellular C-terminus) of rat Neuroligin-3. Mouse: 99% identity (118/119 amino acids identical). Human: 98% identity (116/119 amino acids identical) ~60% identity with Neuroligin-1. ~40% identity with Neuroligin-2.
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-471 was sufficient for detection of Neuroligin 3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Cell Junction | Synapse