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> VGlut3 Antibody

VGlut3 Antibody

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Cat# ASM10231-A680-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - VGlut3 Antibody ASM10231
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGlut3 Monoclonal Antibody, Clone S34-34 (ASM10231). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGlut3 Monoclonal Antibody (ASM10231) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) VGlut3 Antibody (D) Composite.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF
Primary Accession Q7TSF2
Other Accession NP_714947.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat VGlut3 Monoclonal IgG1
Target/Specificity Detects ~65kDa.
Other Names DFNA25 Antibody, Vesicular glutamate transporter 3 Antibody, deafness autosomal dominant 25 Antibody, SLC17A8 Antibody, Solute carrier family 17 member 8 Antibody, VGLUT 3 Antibody, Solute carrier family 17 (sodium dependent inorganic phosphate cotransporter) member 8 Antibody
Clone Names S34-34
Immunogen Fusion protein amino acids 546-588 (cytoplasmic C-terminus) of rat VGlut3
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-397 was sufficient for detection of VGLut3 in 20 µg of CV-1 fibroblast cells (lysate) transfected with VGlut3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasmic Vesicle | Secretory Vesicle | Synaptic Vesicle Membrane | Membrane | Cell Junction | Synapse