RM151 specifically reacts to Histone H3 dimethylated Western Blot of recombinant histone H3.3 (1) ChIP performed on HeLa cells using H3K9me2 Immunocytochemistry of HeLa cells treated with
Product Details Product Name Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM151 Product Description Rabbit monoclonal to Dimethylated Histone H3 Lysine 9, H3K9me2, Histone H3 (dimethyl K9) Catalog No. 31-1059-00 Clone Name RM151 Product Data Sheet Anti-Dimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody Data Sheet RM151 Specificity RM151 reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in histone H3. Immunogen A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys9). Application Western Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt. Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage Western Blot: 0.25 µg/mL – 1 µg/mL;
ChIP: 2 µg/mL - 10 µg/mL;
ICC: 0.5 µg/mL - 2 µg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL. References for Dimethyl-Histone H3 (Lys9) antibody [RM151] Xiong H et al. Inhibition of Histone Methyltransferase G9a Attenuates Noise-Induced Cochlear Synaptopathy and Hearing Loss. J Assoc Res Otolaryngol. (2019), 10.1007/s10162-019-00714-6. ICC, IHC; Human. Read more (PubMed: 30710318) . Product Images
at Lysine 9 (K9me2). No cross reactivity with non
-modified Lysine 9 (H3 1-19), monomethylated
Lysine 9 (K9me1), trimethylated Lysine 9
(K9me3), or other methylations in Histone H3.
and acid extracts of HeLa cells (2), using RM151
at 0.5 µg/mL, showed a band of histone H3
dimethylated at Lysine 9 (K9me2) in HeLa cells.
antibody (RM151, 5 µg). Real-time PCR was
performed using primers specific to the gene
indicated.
sodium butyrate, using Dimethyl-Histone H3
(Lys9) Rabbit mAb RM151 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
Anti-Dimethyl Histone H3 (Lys9) rabbit monoclonal antibody [RM151]
![Anti-Dimethyl Histone H3 (Lys9) rabbit monoclonal antibody [RM151]](https://www.clinisciences.com/en/upload/-img-lapin1.png)
Cat# 31-1059-00
Size : 100ug
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Rabbit monoclonal to Dimethylated Histone H3 Lysine 9, H3K9me2, Histone H3 (dimethyl K9)
25 µg Trial Size, 100 µg Full Size
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