Immunohistochemical staining of formalin fixed and paraffin embedded human Glioblastoma Multiforme tissue sections with or without EGFRvIIII using anti-EGFRvIII antibody (Clone RM419) at 1:100 dilution. Western Blot analysis of DKMG (EGFRvIII positive), U118MG, A431, and HeLa whole cell lysates, using anti-EGFRvIII antibody (Clone RM419) and anti-EGFRct (Clone RM294) at 1:1000 dilution. Flow cytometry analysis of DKMG and 293 cells, using anti-EGFRvIII antibody (Clone RM419) at 1:50 dilution. ELISA analysis showing RM419 does not react to wild type EGFR protein. The plate was coated with recombinant EGFR variant III and EGFRwt proteins. A serial dilution of anti-EGFRvlll (Clone RM419) or anti-EGFR (Clone RM229) antibodies were used.
Product Details Product Name Anti-EGFR Variant III Rabbit Monoclonal Antibody, Clone RM419 Product Description Rabbit monoclonal to Epidermal Growth Factor Receptor Variant III Catalog No. 31-1305-00
31-1305-02 Clone Name RM419 Product Data Sheet Anti-EGFR Variant III Rabbit Monoclonal Antibody Data Sheet RM419
Biotinylated Anti-EGFR Variant III Rabbit Monoclonal Antibody Data Sheet RM419 Specificity RM419 reacts to human Epidermal Growth Factor Receptor Variant III. No cross reactivity with wild type EGF receptor. Immunogen A peptide corresponding to epidermal growth factor receptor variant III (EGFRvIII) Application IHC, WB, ELISA, FC
Species Reactivity Human
Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µL;
Biotinylated 50 µL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage IHC: 1:100 – 1:200 dilution;
FC: 1:10 – 1:100 dilution;
WB: 1:1000 - 1:2000 dilution;
ELISA: 1:100 – 1:1000 dilution. Keywords . Product Images
Anti-EGFR Variant III (EGFRvIII) Rabbit Monoclonal Antibody [RM419]
![Anti-EGFR Variant III (EGFRvIII) Rabbit Monoclonal Antibody [RM419]](https://www.clinisciences.com/en/upload/-img-lapin1.png)
Cat# 31-1305-00
Size : 100uL
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Rabbit monoclonal to EGF Receptor Variant III,
EGFRvIII, EGFRv3
100 µL Standard/50 µL Biotin Conjugate