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Anti-Wnt6 Polyclonal Antibody

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Cat# NB-64-164112-50uL

Size : 50uL

Brand : Neo Biotech


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Anti-Wnt6 Polyclonal Antibody

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Synonyms:

Catalog No. TMAB-14195 Copy Product Info
Anti-Wnt6 Polyclonal Antibody is a Rabbit antibody targeting Wnt6. Anti-Wnt6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-Wnt6 Polyclonal Antibody is a Rabbit antibody targeting Wnt6. Anti-Wnt6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat)
Verified Activity
1. Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Wnt6 Polyclonal Antibody, Unconjugated (TMAB-14195) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: MKN45 (Human) Cell Lysate at 30 μg
Primary: Anti-Wnt6 (TMAB-14195) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 42 kD
3. Sample: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Primary: Anti-Wnt6 (TMAB-14195) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 48 kD
Application
WB
IHC-P
IHC-Fr
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted, extracellular space, extracellular matrix.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundWnt6 is a member of the WNT protein family. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt6 is overexpressed in cervical and breast cell carcinomas and is co-expressed with Wnt10a in colorectal cancer cell lines.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Wnt6
Antigen Species
Human
Gene Name
WNT6
Gene ID
7475
Protein Name
Protein Wnt-6
Uniprot ID
Q9Y6F9
Chemical Properties
Molecular WeightTheoretical: 38 kDa. Actual: 42-48 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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