Caspase 4 (CASP4) (1-270) Mouse Monoclonal Antibody [Clone ID: 4B9]
CAT#: AM26585AF-N
Caspase 4 (CASP4) (1-270) mouse monoclonal antibody, clone 4B9, Azide Free
Specifications
Product Data | |
Clone Name | 4B9 |
Applications | WB |
Recommended Dilution | Western blot: 1 µg/ml for chemiluminescence detection system. For details see protocol below. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Recombinant protein corresponding to N-terminal amino acids (1-270 aa) of human TX |
Specificity | This antibody reacts with caspase-4 (43 kDa) on Western blotting using total cell lysate from U937, HL60 and HUC-Fm (Human primary cultured fibroblast), and also reacts with 44 kDa of myc-tagged-TX expressed in 293T cell. Occasionally, uni dentified 68 kDa band might be detected on western blotting in some cell lines. |
Formulation | PBS containing 50% glycerol, pH 7.2. No preservative is contained. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Gene Name | caspase 4 |
Database Link | |
Background | The interleukin-1 β converting enzyme (ICE)/CED-3 family pr oteases has been implicated in playing a fundamental role in programmed cell death. TX is a member of the ICE/ CED-3 gene family encoding a cysteine protease that ha s a more than 50% sequence homology with ICE, especially in the region encoding the mature p20 and p10 ICE subunits and 30% sequence homology with Nedd-2/Ich-1L and CED-3. TX is able to cleave itself and the p30 IC E precursor and induces apoptosis in transfected cells1). TX is also a member of the caspase (CASP) family, CASP-4. An early biochemical event that occurs apoptosis in many cell types is the proteolytic cleavage of poly (ADP-ribose) polymerase (PARP), a nuclear enzyme in volved in DNA repair. The several mammalian ICE homologues, ICE, TX, Nedd-2/Ich-1L and CPP32, ar e capable of cleaving PARP. |
Synonyms | CASP4, ICH2, ICE(rel)-II, Protease ICH-2, Protease TX |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting |
Reference Data |
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