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> CD31 (PE)

CD31 (PE)

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Cat# C2383-02D-PE-100ul

Size : 100ul

Brand : US Biological


C2383-02D-PE CD31 (PE)

Clone Type
Monoclonal
Host
mouse
Source
sheep
Isotype
IgG2a
Grade
Affinity Purified
Applications
FC IP
Crossreactivity
Bo Gt Sh
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes. CD31 is also highly expressed by ovine endothelial cells.
Reported to inhibit homotypic leukocyte aggregation induced by anti CD43 antibodies (2). Removal of sodium azide is recommended prior to use in fuctional assays.

Applications:
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Optimal dilution determined by the researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the SP2-0/Ag14 mouse myeloma cell line.

Recommended Negative Control:
I1904-83P1: IgG2a Murine Negative Control

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: 6A279 (CO.3E1D4)|Host: mouse|Source: sheep|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Ovine leukocytes|Specificity: Recognizes sheep CD31, also known as PECAM-1|Species Crossreactivity: bovine and goat||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Ovine leukocytes
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes sheep CD31, also known as PECAM-1|Species Crossreactivity: bovine and goat
References
1. Brodersen, R. et al. (1998). Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species: Vet. Immunol. Immunopathol. 64: 1-13. 2. Pintado. C. O. et al. (1995). A monoclonal antibody to an ovine gp130 molecule inhibits homotypic aggregation induced by anti CD43 monoclonal antibodies of ruminant leukocytes. Immunol. Lett. 45: 81-85.