CD81 Recombinant Monoclonal Mouse Antibody (2304.81) – Biotium Choice
Cat# P014-1MG
Size : 200UL
Brand : Biotium
CD81 Recombinant Monoclonal Mouse Antibody (2304.81) - Biotium Choice
CD81 (clone 2304.81) is a validated recombinant mouse monoclonal antibody that recognizes the human tetraspanin protein CD81. The antibody belongs to the Biotium Choice list of select antibodies that have been formulated and validated in-house for optimal performance.
Methanol-fixed MCF7 cells were stained with 1 ug/mL recombinant monoclonal mouse anti-CD81 (2304.81), detected with CF®568 goat anti-mouse secondary antibody and imaged by confocal microscopy.
Recombinant CD81(2304.81) antibody was conjugated to CF®568 and used to stain MCF-7 cell-derived EVs (left panel). EV staining was compared to the antibody alone in buffer (right panel). Staining was detected in the B610 (PI) channel.
Western detection of human CD81 in MCF-7 cell and EV lysates using CD81 Recombinant Mouse Monoclonal Antibody (2304.81) followed by goat anti-mouse CF®680. M: 2 uL Peacock™ Plus Prestained Protein Marker. 10 ug of MCF-7 cell lysate and 1 ug of MCF-7 EV lysate were loaded. The blot was imaged on a LI-COR Odyssey® infrared imaging system in the 700 channel.
Recombinant CD81(2304.81) antibody was conjugated to CF®405M and used to stain the surface of Jurkat cells (blue). Isotype control antibody conjugated to CF®405M is shown in gray. Staining was detected in the V450 (Pacific Blue) channel. 
Recombinant CD81(2304.81) antibody was conjugated to CF®488A and used to stain the surface of U2-OS cells (green). Isotype control antibody conjugated to CF®488A is shown in gray. Staining was detected in the B525 (FITC) channel. 
Recombinant CD81(2304.81) antibody was conjugated to CF®568 and used to stain the surface of MCF-7 cells (orange). Unstained cells are shown in gray. Staining was detected in the Y585 (PE) channel. 
Recombinant CD81(2304.81) antibody was conjugated to CF®583R and used to stain the surface of MCF-7 cells (red). Unstained cells are shown in gray. Staining was detected in the Y610 (mCherry) channel. 
Recombinant CD81(2304.81) antibody was conjugated to CF®647Plus and used to stain the surface of MCF-7 cells (pink). Isotype control antibody conjugated to CF®647Plus is shown in gray. Staining was detected in the R660 (APC) channel. 
Recombinant CD81(2304.81) antibody was conjugated to APC and used to stain the surface of MCF-7 cells (pink). Isotype control antibody conjugated to APC is shown in gray. Staining was detected in the R660 (APC) channel. 
Recombinant CD81(2304.81) antibody was conjugated to CF®700 and used to stain the surface of MCF-7 cells (teal). Isotype control antibody conjugated to CF®700 is shown in gray. Staining was detected in the R712 (APC-700) channel. 
Recombinant CD81(2304.81) antibody was conjugated to RPE-Astral™775 and used to stain the surface of MCF-7 cells (teal). Isotype control antibody conjugated to RPE-Astral™775 is shown in gray. Staining was detected in the Y763 channel. Product Description
CD81 (clone 2304.81) is a recombinant mouse monoclonal antibody that recognizes the human tetraspanin protein CD81. This antibody belongs to the Biotium Choice list of select antibodies that have been formulated and validated in-house for optimal performance. This antibody has been validated in flow cytometry and immunofluorescence microscopy. The antibody is available unlabeled or conjugated to CF® Dyes and Astral™ tandem dyes.
CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspanin transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.
Tetraspanin proteins CD9, CD63, and CD81, are the most common proteins used as exosome markers. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of exosomes, but their expression levels vary depending on the cell type of origin.
As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody.
Exosome Stains and Antibodies
Biotium also offers ExoBrite™ CD81 Flow Antibody and ExoBrite™ CD81 Western Antibody that are validated for optimal detection of isolated exosomes by flow cytometry or western blotting. Learn more about our products for exosome research.
Discover Sharper Signals and Unmatched Panel Flexibility with Biotium Choice Antibodies
Powered by CF® Dyes and Astral Leap™ Tandem Dyes
Biotium Choice antibodies are carefully curated and validated in-house to offer exceptional signal-to-noise. Labeled with our advanced CF® Dyes and Astral Leap™ tandem dyes, they are our top-recommended antibodies for flow cytometry and other applications.
Biotium Choice Antibody Features
- Robust and validated clones against common targets
- Developed and optimized for flow cytometry and other applications
- Conjugated to bright, photostable CF® Dyes for superior signal and clarity
- Also available with Astral Leap™ tandem dyes for expanding multiplexing
- New antibody clones and dye conjugates continuously in development
View our full catalog of Biotium Choice antibodies
Identifying Biotium validated antibodies
Biotium scientists validate and optimize select antibodies in our catalog, marked with a check mark next to the product name. This symbol indicates the antibody is highly recommended for the specified application(s).
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| Conjugation | Ex/Em | Conc. | Size | Catalog No. | Dye Features |
|---|---|---|---|---|---|
| CF®405M | 416/452 nm | 100 ug/mL | 25 tests (125 uL) | P014-405M-125 | CF®405M Features |
| 100 tests (500 uL) | P014-405M-500 | ||||
| CF®488A | 490/516 nm | 100 ug/mL | 25 tests (125 uL) | P014-488A-125 | CF®488A Features |
| 100 tests (500 uL) | P014-488A-500 | ||||
| CF® 568 | 562/584 nm | 100 ug/mL | 25 tests (125 uL) | P014-568-125 | CF®568 Features |
| 100 tests (500 uL) | P014-568-500 | ||||
| CF® 583R | 585/609 nm | 100 ug/mL | 25 tests (125 uL) | P014-583R-125 | CF®583R Features |
| 100 tests (500 uL) | P014-583R-500 | ||||
| RPE-Astral™616 | 496, 546, 566/617 nm | 100 ug/mL | 25 tests (125 uL) | P014-R616-125 | RPE-Astral™616 Features |
| 100 tests (500 uL) | P014-R616-500 | ||||
| CF® 647Plus | 652/668 nm | 100 ug/mL | 25 tests (125 uL) | P014-647P-125 | CF®647Plus Features |
| 100 tests (500 uL) | P014-647P-500 | ||||
| CF®700 | 696/721 nm | 100 ug/mL | 25 tests (125 uL) | P014-700-125 | CF®700 Features |
| 100 tests (500 uL) | P014-700-500 | ||||
| RPE-Astral™775 | 496, 546, 565/774 nm | 100 ug/mL | 25 tests (125 uL) | P014-R775-125 | RPE-Astral™775 Features |
| 100 tests (500 uL) | P014-R775-500 | ||||
| R-PE | 496, 546, 565/576 nm | 100 ug/mL | 25 tests (125 uL) | P014-RPE-125 | |
| 100 tests (500 uL) | P014-RPE-500 | ||||
| APC | 651/660 nm | 100 ug/mL | 25 tests (125 uL) | P014-APC-125 | |
| 100 tests (500 uL) | P014-APC-500 | ||||
| Purified (BSA-free) | N/A | 5 mg/mL | 1 mg (200 uL) | P014-1MG | |
| 5 mg (1 mL) | P014-5MG |
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