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> Horse Integrin Alpha 2B (ITGa2B) ELISA Kit

Horse Integrin Alpha 2B (ITGa2B) ELISA Kit

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Cat# orb1291317-96Tests

Size : 96T

Brand : Biorbyt


Horse Integrin Alpha 2B (ITGa2B) ELISA Kit

SKU: orb1291317
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Description

The Horse Integrin Alpha 2B (ITGa2B) ELISA Kit is designed to quantify Integrin Alpha 2B (ITGa2B) in equine tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.058 ng/mL.

Research Area

Signal Transduction

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse ITGa2B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse ITGa2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse ITGa2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse ITGa2B in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityEquine
Sample TypesTissue homogenates, cell lysates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.058 ng/mL
Concentration10 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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CD41, CD41B, ITG-A2B, GTA, GP2B, GPIIb, HPA3, Platelet Glycoprotein IIb Of IIb/IIIa Complex, Antigen CD41

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol