- A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-human COR antibody.
- Two vails Lyophilized Standard -- Dilute one of the standards at dilution series, read the OD values, and then draw a standard curve.
- Anti-human COR Antibody (100 x concentrate) 1 x 60 μl-- Act as the detection antibody.
- Antibody Diluent 1 x 10 ml -- Dilute the COR antibody solution.
- HRP-conjugate (100 x concentrate) 1 x 120 μl-- Bind to the COR antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
- HRP-conjugate Diluent 1 x 20 ml -- Dilute the HRP-conjugate solution.
- Sample Diluent 2 x 20 ml -- Dilute the standard and sample solutions. Wash Buffer (25 x concentrate) 1 x 20 ml -- Wash away unbound or free substances.
- TMB Substrate 1 x 10 ml -- HRP catalyzes the TMB, eliciting a chromogenic reaction.
- Stop Solution 1 x 10 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
Human Cortisol ELISA Kit
Cat# CSB-E05111h-96T
Size : 96T
Brand : Cusabio
| Size | 96T,5×96T,10×96T |
Product Details
Abbreviation
COR
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates, saliva.
Detection Range
3.12 ng/ml-200 ng/ml.
Sensitivity
1.56 ng/ml.
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Linearity
Recovery
Typical Data
Materials provided
Materials not provided
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Citations
- ЕМ Франциянц, ВА Бандовкина,Research'n Practical Medicine Journal,2026
- Эффективность комплексного применения лечебных физических факторов у больных истинной экземой, ассоциированной с метаболическим синдромом АА Михайлова,Thesis & Dissertation,/
- N Xiao, X Zhang, CLW Chan,Stress and Health,2025
- KN Tran,/,/
- JY Lee,Yonsei medical journal,2024
- M Yu,/,2023
- D Antypa,Neurobiology of learning and memory,2023
- F Yang,The Science of the total environment,2023
- YB Lee,Scientific reports,2023
- E Koutentaki,Healthcare,2023
Customer Reviews and Q&A
Average Rating:
Sample type: Biological fluids(Saliva)
Sample species: Human
Review: Cortisol responses to the Trier Social Stress Test by bullying group. Cortisol was measured in nanograms per milliliter (ng/ml) and was log-transformed in this figure. The figure shows means for cortisol levels, for physical bullied, verbal bullied, relational bullied, the total bullied and ...
By Anonymous
Sample type: Plasma (anticoagulant)
Sample species: Human
Review: Cortisol levels in Experiment 2. Food, n = 21; Fasting, n = 24. b Cortisol levels in Experiment 4. Food, n = 22; Fasting, n = 23. Blue dots and red dots represent individuals in the food group and fasting group, respectively.
By Anonymous
Sample type: Biological fluids(saliva)
Sample species: Human
Sample dilution: 1：4
Review: I used csb-E05111h to detect human salivary cortisol, the OD value is 1.5285, the concentration is 23.09, the overall standard curve of the kit is better and more stable.
By Anonymous
■ Q&A
Q:
I would like to know if corticosterone also affects to OD value.
A:
Thanks for your inquiry.
This kit is specific to Cortisol.It has no correlation with corticosterone and the corticosterone does not affect OD value.
This kit is specific to Cortisol.It has no correlation with corticosterone and the corticosterone does not affect OD value.
Q:
I hope you would be able to advice me which kit would
be suitable for me to purchase in order to conduct human salivary
ELISA assay on cortisol. Sample size is 60 and running in
duplicates.
A:
Thanks for your inquiry.
We have CSB-E05111h available. It is based on competitive method. It is suitable to test human salivary. Pls folow actual test result.
One 96T kit can test 43 samples if you run in duplicates.
We have CSB-E05111h available. It is based on competitive method. It is suitable to test human salivary. Pls folow actual test result.
One 96T kit can test 43 samples if you run in duplicates.