| Short Description : | This P2RX7 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Applications: | ELISA |
| Reactivity: | Human |
| Sensitivity: | 0.112ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
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| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Widely expressed with highest levels in brain and immune tissues. Isoform B: Predominant form in many tissues. |
| Post Translational Modifications | Phosphorylation results in its inactivation. ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel. Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface. Palmitoylation prevents channel desensitization by physically anchoring the palmitoylated groups to the membrane. |
| Function | ATP-gated nonselective transmembrane cation channel that requires high millimolar concentrations of ATP for activation. Upon ATP binding, it rapidly opens to allow the influx of small cations Na(+) and Ca(2+), and the K(+) efflux. Also has the ability to form a large pore in the cell membrane, allowing the passage of large cationic molecules. In microglia, may mediate NADPH transport across the plasma membrane. In immune cells, P2RX7 acts as a molecular sensor in pathological inflammatory states by detecting and responding to high local concentrations of extracellar ATP. In microglial cells, P2RX7 activation leads to the release of pro-inflammatory cytokines, such as IL-1beta and IL-18, through the activation of the NLRP3 inflammasome and caspase-1. Cooperates with KCNK6 to activate NLRP3 inflammasome. Activates death pathways leading to apoptosis and autophagy. Activates death pathways leading to pyroptosis. Isoform B: Shows ion channel activity but no macropore function. Isoform H: Non-functional channel. Isoform J: Non-functional channel. |
| Protein Name | P2x Purinoceptor 7P2x7Atp ReceptorP2z ReceptorPurinergic Receptor |
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| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative ELISA Names | P2x Purinoceptor 7 ELISA kitP2x7 ELISA kitAtp Receptor ELISA kitP2z Receptor ELISA kitPurinergic Receptor ELISA kitP2RX7 ELISA kit |
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Information sourced from Uniprot.org