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IPTG

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Cat# C0040

Size : 5g

Brand : Canvax Biotech


IPTG reagent vial by Canvax, inducer of lac operon for cloning and protein expression

IPTG

High-purity IPTG (>99%), dioxane-free inducer of lac operon for cloning and protein expression in E. coli.

IPTG (Isopropyl β-D-1-thiogalactopyranoside) is a molecular biology reagent used to induce expression of genes under the lac promoter system. It serves as a non-metabolizable analog of lactose, activating transcription in bacterial cultures.

This compound is highly pure and stable, ensuring consistent induction of target genes in recombinant protein expression experiments.

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(2 customer reviews)

Detailed information:

Advantages & Features

  • High purity: >99% confirmed by HPLC.

  • Dioxane-free: no disruption of normal cell function.

  • Stable inducer: not degraded by β-galactosidase.

  • Reliable: reproducible induction of gene expression.

Specifications

  • CAS Number: 367-93-1
  • Chemical Formula: C9H18O5S
  • Molecular Weight: 238.30
  • Purity (HPLC)(on dry basis): >99.0%
  • Melting point: 110 – 114 °C
  • Identity (IR): conforms to structure
  • Solubility: soluble in water and methanol
  • Heavy metals (Pb): <5ppm
  • 1,4-Dioxane: Not detected
  • pH (5% in water): 5.0 – 7.0
  • Water content (Karl Fischer): <1.0%

Includes

– 5 g IPTG

Download documentation

Applications

  • Blue/white colony screening.

  • Induction of gene expression under lac promoter.

  • Protein expression in recombinant E. coli.

Tables & Figures

Quality Control

  • Purity tested by HPLC (>99%).

  • Verified dioxane-free.

  • Tested for solubility and heavy metals.

Advice

  • Prepare fresh sterile stock solutions.

  • Store aliquots to avoid repeated freeze-thaw.

Storage, Shipping & Guarantee

  • Shipped in: Gel pack.
  • Storage: 4 ºC .

Citations

  • Pacheco-Garcia, J. L., Anoz-Carbonell, E., Loginov, D. S., Vankova, P., Salido, E., Man, P., … & Pey, A. L. (2022). Different phenotypic outcome due to site-specific phosphorylation in the cancer-associated NQO1 enzyme studied by phosphomimetic mutations. Archives of Biochemistry and Biophysics, 109392.
  • Pacheco-García, J. L., Loginov, D. S., Naganathan, A. N., Vankova, P., Cano-Muñoz, M., Man, P., & Pey, A. L. (2022). Perturbation analysis in the N-terminal domain of hPGK1: a biophysical, structural and statistical mechanical study.
  • Pacheco-Garcia, J. L., Loginov, D. S., Anoz-Carbonell, E., Vankova, P., Palomino-Morales, R., Salido, E., … & Pey, A. L. (2022). Allosteric Communication in the Multifunctional and Redox NQO1 Protein Studied by Cavity-Making Mutations. Antioxidants, 11(6), 1110.
  • Pacheco-García, J. L., Cano-Muñoz, M., Sánchez-Ramos, I., Salido, E., & Pey, A. L. (2020). Naturally-Occurring Rare Mutations Cause Mild to Catastrophic Effects in the Multifunctional and Cancer-Associated NQO1 Protein. Journal of Personalized Medicine, 10(4), 207.
  • Blanco, S., Peralta, S., Morales, M. E., Martínez-Lara, E., Pedrajas, J. R., Castán, H., … & Ruiz, M. A. (2020). Hyaluronate Nanoparticles as a Delivery System to Carry Neuroglobin to the Brain after Stroke. Pharmaceutics, 12(1), 40.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

Synonym(s)

IPTG inducer, Isopropyl β-D-1-thiogalactopyranoside, Lac operon inducer, Gene expression inducer IPTG, Protein expression inducer

Also known as:

  • Spanish: IPTG, Isopropil β-D-1-tiogalactopiranósido, Inductor del operón lac
  • French: IPTG, Isopropyl β-D-1-thiogalactopyranoside, Inducteur de l’opéron lac
  • German: IPTG, Isopropyl-β-D-1-thiogalactopyranosid, Lac-Operon-Induktor
  • Italian: IPTG, Isopropil β-D-1-tiogalattopiranoside, Induttore operone lac

FAQs

Q: What is the purity of IPTG Canvax?
A: >99% confirmed by HPLC.

Q: Is this IPTG dioxane-free?
A: Yes, no 1,4-dioxane detected.

Q: What is the main application?
A: Induction of gene expression under the lac promoter and blue/white screening.

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