Isotype Control, Mouse IgG1 Kappa (IGG1/1331), RPE Conjugate, 0.1 mg/mL
Cat# BNCR1331-1ML
Size : 1mL
Brand : Biotium
Isotype Control, Mouse IgG1 Kappa (IGG1/1331), RPE Conjugate, 0.1 mg/mL
Flow cytometry of Jurkat cells stained with CD81 Mouse Monoclonal Antibody (2304.81), CF®405M Conjugate (dotted blue line) and isotype control antibody conjugated to CF®405M (blue histogram). Unstained cells are shown in gray. Staining was detected in the V450 (Pacific Blue) channel.
Flow cytometry of MCF-7 cells stained with Recombinant CD63 Mouse Monoclonal Antibody (2304.63), CF®488A Conjugate (dotted green line) and isotype control antibody conjugated to CF®488A (green histogram). Unstained cells are shown in gray. Staining was detected in the B525 (FITC) channel.
Flow cytometry of MCF-7 cells stained with Recombinant CD9 Mouse Monoclonal Antibody (2310.9), CF®568 Conjugate (dotted orange line) and isotype control antibody conjugated to CF®568 (orange histogram). Unstained cells are shown in gray. Staining was detected in the Y585 (PE) channel.
Flow cytometry of lymphocyte-gated PBMCs stained with CD45 Mouse Monoclonal Antibody (2D1), PerCP Conjugate (dotted magenta line) and isotype control antibody conjugated to PerCP (magenta histogram). Unstained cells are shown in gray. Staining was detected in the B690 (PerCP) channel.
Flow cytometry of Jurkat cells stained with CD3 Mouse Monoclonal Antibody (SK7), APC Conjugate (dotted pink line) and isotype control antibody conjugated to APC (pink histogram). Unstained cells are shown in gray. Staining was detected in the R660 (APC) channel.
Flow cytometry of MCF-7 cells stained with Recombinant CD9 Mouse Monoclonal Antibody (2310.9), CF®700 Conjugate (dotted teal line) and isotype control antibody conjugated to CF®700 (teal histogram). Unstained cells are shown in gray. Staining was detected in the R712 (APC-700) channel.
Flow cytometry of Jurkat cells stained with CD3 Mouse Monoclonal Antibody (SK7), CF®820 Conjugate (dotted teal line) and isotype control antibody conjugated to CF®820 (teal histogram). Unstained cells are shown in gray. Staining was detected in the IR840 channel.
Flow cytometry of lymphocyte-gated PBMCs stained with CD45 Mouse Monoclonal Antibody (2D1), PerCP Conjugate (dotted magenta line) and isotype control antibody conjugated to PerCP (magenta histogram). Unstained cells are shown in gray. Staining was detected in the B690 (PerCP) channel.
Flow cytometry of MCF-7 cells stained with Recombinant CD9 Mouse Monoclonal Antibody (2310.9), RPE-Astral™775 Conjugate (dotted teal line) and isotype control antibody conjugated to RPE-Astral™775 (teal histogram). Unstained cells are shown in gray. Staining was detected in the Y763 channel.
Primary & Secondary Antibody Conjugates
Protocol: Fluorescent Western Blotting
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host
Troubleshooting Tips for Fluorescence Staining
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Tech Tip: Fluorescence Bleed-Through and Cross-Talk
Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331)
Non-reactive antibody to use as a negative control for mouse IgG1 primary antibodies. Available with CF® Dyes and other labels.
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Flow cytometry of Jurkat cells stained with CD81 Mouse Monoclonal Antibody (2304.81), CF®405M Conjugate (dotted blue line) and isotype control antibody conjugated to CF®405M (blue histogram). Unstained cells are shown in gray. Staining was detected in the V450 (Pacific Blue) channel.
Flow cytometry of MCF-7 cells stained with Recombinant CD63 Mouse Monoclonal Antibody (2304.63), CF®488A Conjugate (dotted green line) and isotype control antibody conjugated to CF®488A (green histogram). Unstained cells are shown in gray. Staining was detected in the B525 (FITC) channel.
Flow cytometry of MCF-7 cells stained with Recombinant CD9 Mouse Monoclonal Antibody (2310.9), CF®568 Conjugate (dotted orange line) and isotype control antibody conjugated to CF®568 (orange histogram). Unstained cells are shown in gray. Staining was detected in the Y585 (PE) channel.
Flow cytometry of lymphocyte-gated PBMCs stained with CD45 Mouse Monoclonal Antibody (2D1), PerCP Conjugate (dotted magenta line) and isotype control antibody conjugated to PerCP (magenta histogram). Unstained cells are shown in gray. Staining was detected in the B690 (PerCP) channel.
Flow cytometry of Jurkat cells stained with CD3 Mouse Monoclonal Antibody (SK7), APC Conjugate (dotted pink line) and isotype control antibody conjugated to APC (pink histogram). Unstained cells are shown in gray. Staining was detected in the R660 (APC) channel.
Flow cytometry of MCF-7 cells stained with Recombinant CD9 Mouse Monoclonal Antibody (2310.9), CF®700 Conjugate (dotted teal line) and isotype control antibody conjugated to CF®700 (teal histogram). Unstained cells are shown in gray. Staining was detected in the R712 (APC-700) channel.
Flow cytometry of Jurkat cells stained with CD3 Mouse Monoclonal Antibody (SK7), CF®820 Conjugate (dotted teal line) and isotype control antibody conjugated to CF®820 (teal histogram). Unstained cells are shown in gray. Staining was detected in the IR840 channel.
Flow cytometry of lymphocyte-gated PBMCs stained with CD45 Mouse Monoclonal Antibody (2D1), PerCP Conjugate (dotted magenta line) and isotype control antibody conjugated to PerCP (magenta histogram). Unstained cells are shown in gray. Staining was detected in the B690 (PerCP) channel.
Flow cytometry of MCF-7 cells stained with Recombinant CD9 Mouse Monoclonal Antibody (2310.9), RPE-Astral™775 Conjugate (dotted teal line) and isotype control antibody conjugated to RPE-Astral™775 (teal histogram). Unstained cells are shown in gray. Staining was detected in the Y763 channel.View Spectra Viewer
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Product Description
This is a non-reactive mouse monoclonal IgG1 kappa antibody labeled with one of our bright and photostable CF® Dyes and other labels. This antibody may serve as a control for a mouse monoclonal IgG1 antibody under identical experimental conditions. Biotium also carries mouse monoclonal isotype control antibodies for IgG2a Kappa (MG2a-53) and IgG2b Kappa (IGG1/1333).
- Isotype control for antibody-based experiments
- Available with bright and photostable CF® Dyes
- APC, R-PE, RPE-Astral™616, RPE-Astral™775, APC-Astral™813, and biotin conjugates also available
- Suitable for western, flow cytometry, ELISA, immunofluorescence, and immunohistology
CF® Dyes offer exceptional brightness and photostability; learn more about CF® Dyes.
Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331)
| Conjugation | Ex/Em | Size | Catalog No. | Dye Features |
|---|---|---|---|---|
| CF®405S | 411/431 nm | 250 uL (25 ug) | BNC041331-250 | CF®405S Features |
| 1 mL (100 ug) | BNC041331-1ML | |||
| CF®405M | 416/452 nm | 250 uL (25 ug) | BNC051331-250 | CF®405M Features |
| 1 mL (100 ug) | BNC051331-1ML | |||
| CF®405L | 413/547 nm | 250 uL (25 ug) | BNC061331-250 | CF®405L Features |
| 1 mL (100 ug) | BNC061331-1ML | |||
| CF®488A | 562/584 nm | 250 uL (25 ug) | BNC881331-250 | CF®488A Features |
| 1 mL (100 ug) | BNC881331-1ML | |||
| CF®503R | 503/532 nm | 250 uL (25 ug) | BNC501331-250 | CF®503R Features |
| 1 mL (100 ug) | BNC501331-1ML | |||
| CF®514 | 516/549 nm | 250 uL (25 ug) | BNC511331-250 | CF®514 Features |
| 1 mL (100 ug) | BNC511331-1ML | |||
| CF®568 | 562/584 nm | 250 uL (25 ug) | BNC681331-250 | CF®568 Features |
| 1 mL (100 ug) | BNC681331-1ML | |||
| CF®575 | 575/601 nm | 250 uL (25 ug) | BNC571331-250 | |
| 1 mL (100 ug) | BNC571331-1ML | |||
| CF®583R | 585/609 nm | 250 uL (25 ug) | BNC581331-250 | CF®583R Features |
| 1 mL (100 ug) | BNC581331-1ML | |||
| CF®594 | 593/615 nm | 250 uL (25 ug) | BNC941331-250 | CF®594 Features |
| 1 mL (100 ug) | BNC941331-1ML | |||
| RPE-Astral™616 | 496, 546, 566/617 nm | 250 uL (25 ug) | BNC161331-250 | RPE-Astral™616 Features |
| 1 mL (100 ug) | BNC161331-1ML | |||
| CF®640R | 642/663 nm | 250 uL (25 ug) | BNC401331-250 | CF®640R Features |
| 1 mL (100 ug) | BNC401331-1ML | |||
| CF®647 | 652/668 nm | 250 uL (25 ug) | BNC471331-250 | CF®647 Features |
| 1 mL (100 ug) | BNC471331-1ML | |||
| CF®647Plus | 652/668 nm | 250 uL (25 ug) | BNC641331-250 | CF®647 Features |
| 1 mL (100 ug) | BNC641331-1ML | |||
| CF®660C | 667/685 nm | 250 uL (25 ug) | BNC601331-250 | CF®660C Features |
| 1 mL (100 ug) | BNC601331-1ML | |||
| CF®700 | 696/721 nm | 250 uL (25 ug) | BNC001331-250 | CF®700 Features |
| 1 mL (100 ug) | BNC001331-1ML | |||
| CF®820 | 822/835 nm | 250 uL (25 ug) | BNC201331-250 | CF®820 Features |
| 1 mL (100 ug) | BNC201331-1ML | |||
| CF®870 | 876/896 nm | 250 uL (25 ug) | BNC701331-250 (Discontinued) | CF®870 Features |
| 1 mL (100 ug) | BNC701331-1ML (Discontinued) | |||
| RPE-Astral™775 | 496, 546, 565/774 nm | 250 uL (25 ug) | BNC751331-250 | RPE-Astral™775 Features |
| 1 mL (100 ug) | BNC751331-1ML | |||
| APC-Astral™813 | 633, 638/813 nm | 250 uL (25 ug) | BNC131331-250 | APC-Astral™813 Features |
| 1 mL (100 ug) | BNC131331-1ML | |||
| R-PE | 496, 546, 565/576 nm | 250 uL (25 ug) | BNCR1331-250 | |
| 1 mL (100 ug) | BNCR1331-1ML | |||
| PerCP | 482/675 nm | 1 mL (100 ug) | BNCP1331-1ML | |
| 250 uL (25 ug) | BNCP1331-250 | |||
| APC | 651/660 nm | 250 uL (25 ug) | BNCA1331-250 | |
| 1 mL (100 ug) | BNCA1331-1ML | |||
| Biotin | N/A | 250 uL (25 ug) | BNCB1331-250 | |
| 1 mL (100 ug) | BNCB1331-1ML | |||
| Purified | N/A | 250 uL (50 ug) | BNUB1331-250 | |
| Purified, BSA-free | N/A | 50 uL (50 ug) | BNUM1331-50 |
Technology
Tech Tips
Product Attributes
Antibody number
#1331
Antibody reactivity (target)
None
Antibody type
Isotype Control
Host species
Mouse
Clonality
Monoclonal
Clone
IGG1/1331
Isotype
IgG1, kappa
Shipping condition
Room temperature
Storage Conditions
Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status
For research use only (RUO)
Antibody application notes
For coating for ELISA, order Ab without BSA, Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Optimal dilution and staining procedure for a specific application should be determined by user, Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Species reactivity
None (isotype control)
Documents, Protocols, SDS and COA
Datasheet
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