Mouse Chemokine Array GS1

Cat# GSM-CHE-1-4

Size : 64SampleKit

Brand : RayBiotech


Mouse Chemokine Array GS1

G-Series Mouse Chemokine Array 1 Kit. Semi-quantitative measurement of 25 mouse chemokines. Suitable for all liquid sample types.

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Product Description

Specifications

Size16 Sample Kit, 32 Sample Kit, 64 Sample Kit
SpeciesMouse
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeSemi-Quantitative
Number of Targets Detected25
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Research AreaInflammation, Chemokine Biology
Shipping TypeBlue ice
Storage-20°C

Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

Product Features

  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation

Target Names

Scroll over each target protein for more information
6Ckine
(CCL21)
  • Gene Symbol: CCL21
  • Accession #: P86792
  • ELISA Kit: RayBio Mouse 6Ckine ELISA
BLC
(CXCL13)
  • Gene Symbol: CXCL13
  • Accession #: O55038
  • ELISA Kit: RayBio Mouse BLC ELISA
CTACK
(CCL27)
  • Gene Symbol: CCL27
  • Accession #: Q9Z1X0
  • ELISA Kit: RayBio Mouse CTACK ELISA
CXCL16
  • Gene Symbol: CXCL16
  • Accession #: Q5F2D5
  • ELISA Kit: RayBio Mouse CXCL16 ELISA
Eotaxin-1
(CCL11)
  • Gene Symbol: CCL11
  • Accession #: P48298
  • ELISA Kit: RayBio Mouse Eotaxin-1 ELISA
Eotaxin-2
(MPIF-2/CCL24)
  • Gene Symbol: Ccl24
  • Accession #: Q9JKC0
  • ELISA Kit: RayBio Mouse Eotaxin-2 ELISA
Fractalkine
(CX3CL1)
  • Gene Symbol: CX3CL1
  • Accession #: O35188
  • ELISA Kit: RayBio Mouse Fractalkine ELISA
I-309
(TCA-3/CCL1)
  • Gene Symbol: CCL1
  • Accession #: P14098
  • ELISA Kit: RayBio Mouse TCA-3 ELISA
I-TAC
(CXCL11)
  • Gene Symbol: CXCL11
  • Accession #: Q9JHH5
  • ELISA Kit: RayBio Mouse I-TAC ELISA
KC
(CXCL1)
  • Gene Symbol: CXCL1
  • Accession #: P12850
  • ELISA Kit: RayBio Mouse KC ELISA
LIX
  • Gene Symbol: Cxcl5
  • Accession #: P50228
  • ELISA Kit: RayBio Mouse LIX ELISA
MCP-1
(CCL2)
  • Gene Symbol: CCL2
  • Accession #: Q9QYD7
  • ELISA Kit: RayBio Mouse MCP-1 ELISA
MCP-5
  • Gene Symbol: Ccl12
  • Accession #: Q9QYD6
  • ELISA Kit: RayBio Mouse MCP-5 ELISA
MDC
(CCL22)
  • Gene Symbol: CCL22
  • Accession #: O88430
  • ELISA Kit: RayBio Mouse MDC ELISA
MIG
(CXCL9)
  • Gene Symbol: CXCL9
  • Accession #: P18340
  • ELISA Kit: RayBio Mouse MIG ELISA
MIP-1 alpha
(CCL3)
  • Gene Symbol: CCL3
  • Accession #: P14096
  • ELISA Kit: RayBio Mouse MIP-1 alpha ELISA
MIP-1 gamma
  • Gene Symbol: Ccl9
  • Accession #: Q5QNW2
  • ELISA Kit: RayBio Mouse MIP-1 gamma ELISA
MIP-2
  • Gene Symbol: Cxcl2
  • Accession #: P10889
  • ELISA Kit: RayBio Mouse MIP-2 ELISA
MIP-3 alpha
(CCL20)
  • Gene Symbol: CCL20
  • Accession #: O89093
  • ELISA Kit: RayBio Mouse MIP-3 alpha ELISA
MIP-3 beta
(CCL19)
  • Gene Symbol: CCL19
  • Accession #: O70460
  • ELISA Kit: RayBio Mouse MIP-3 beta ELISA
Platelet Factor 4
(CXCL4)
  • Gene Symbol: PF4, CXCL4, SCYB4
  • Accession #: Q9Z126
  • ELISA Kit: RayBio Mouse PF-4 ELISA
RANTES
(CCL5)
  • Gene Symbol: CCL5
  • Accession #: P30882
  • ELISA Kit: RayBio Mouse RANTES ELISA
SDF-1 alpha
(CXCL12 alpha)
  • Gene Symbol: CXCL12
  • Accession #: P40224
  • ELISA Kit: RayBio Mouse SDF-1 alpha ELISA
TARC
(CCL17)
  • Gene Symbol: CCL17
  • Accession #: Q9WUZ6
  • ELISA Kit: RayBio Mouse TARC ELISA
TECK
(CCL25)
  • Gene Symbol: CCL25
  • Accession #: O35903
  • ELISA Kit: RayBio Mouse TECK ELISA

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming a Biological Process
Kit Components
  • Antibody Array Slide/s
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with samples
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAM-CHE-1.gal)
Excel (GSM-CHE-1_Analysis_Tool.xls)

citations

  • Downregulation of SEPTIN5 inhibits prostate cancer progression by increasing CD8+ T cell infiltration

    Species:
    Mouse
    Sample Type:
    RM1 cell
    Year:
    2022
    Journal Name:
    International Journal of Biological Sciences