MS-444 [150045-18-4]
Cat# HY-100685-1ml
Size : 10mM/1mL
Brand : MedChemExpress
MS-444 inhibits the activity of purified smooth muscle myosin light chain kinase (MLCK) with an IC50 value of 10 μM.
For research use only. We do not sell to patients.
MS-444 Chemical Structure
CAS No. : 150045-18-4
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Description |
MS-444 inhibits the activity of purified smooth muscle myosin light chain kinase (MLCK) with an IC50 value of 10 μM. |
IC50 & Target |
IC50: 10 μM (myosin)[1]. |
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In Vitro |
MS-444 is a small molecule RNA-binding protein HuR (ELAVL1) inhibitor. Colorectal cancer (CRC) cells that display HuR overexpression are treated with MS-444 (1-100 μM) for 48 hr with IC50s of 10.98±1.76 μM, 12.84±2.10 μM, 5.60±0.90 μM, 14.21±2.11 μM, and 10.98±1.24 μM for HCT116, HCA-7, RKO, HT-29, and SW480 cells, respectively. Growth inhibition is observed in all CRC lines with IC50 values ranging from 5.60 μM to 14.21 μM with observable effects seen at 10 μM MS-444. Contrasting effects are observed using non-transformed small intestinal (RIE-1 (IC50=40.70±3.53 μM)) and colonic (YAMC (IC50=28.16±3.23 μM)) epithelial cells. Both cell types display properties of normal intestinal epithelial cells and are proficient in 3′UTR AU-rich elements (ARE)-mRNA decay. Both non-transformed cell lines are ~3- to 4-fold less responsive to MS-444-mediated growth inhibition, with IC50 values of 40.70 μM and 28.16 μM (P<0.05)[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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In Vivo |
To test the effects of MS-444 on CRC cell growth in vivo, mice bearing HCT116 cell xenografts receive IP injections of MS-444 (25 mg/kg bw) or vehicle every 48 hr. Over the experiment course, mice do not display any adverse effects and maintained similar weights. Anti-tumor effects of MS-444 are observed with approximately 1.7-fold reduction in tumor size. Mice treated with MS-444 show a marked 2- to 3-fold decrease in microvessel density (MVD), indicating the anti-angiogenic potential of MS-444[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
230.22 |
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Formula |
C13H10O4 |
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CAS No. |
150045-18-4 |
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Appearance |
Solid |
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Color |
Light yellow to brown |
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SMILES |
O=C(C1=C(C)OC=C1C2)C3=C2C(O)=CC=C3O |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
-20°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen) |
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Solvent & Solubility |
In Vitro:
DMSO : 50 mg/mL (217.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) 1-Methyl-2-pyrrolidinone : 20 mg/mL (86.87 mM; Need ultrasonic) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 99.45% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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1-Methyl-2-pyrrolidinone / DMSO | 1 mM | 4.3437 mL | 21.7184 mL | 43.4367 mL | 108.5918 mL |
5 mM | 0.8687 mL | 4.3437 mL | 8.6873 mL | 21.7184 mL | |
10 mM | 0.4344 mL | 2.1718 mL | 4.3437 mL | 10.8592 mL | |
15 mM | 0.2896 mL | 1.4479 mL | 2.8958 mL | 7.2395 mL | |
20 mM | 0.2172 mL | 1.0859 mL | 2.1718 mL | 5.4296 mL | |
25 mM | 0.1737 mL | 0.8687 mL | 1.7375 mL | 4.3437 mL | |
30 mM | 0.1448 mL | 0.7239 mL | 1.4479 mL | 3.6197 mL | |
40 mM | 0.1086 mL | 0.5430 mL | 1.0859 mL | 2.7148 mL | |
50 mM | 0.0869 mL | 0.4344 mL | 0.8687 mL | 2.1718 mL | |
60 mM | 0.0724 mL | 0.3620 mL | 0.7239 mL | 1.8099 mL | |
80 mM | 0.0543 mL | 0.2715 mL | 0.5430 mL | 1.3574 mL | |
DMSO | 100 mM | 0.0434 mL | 0.2172 mL | 0.4344 mL | 1.0859 mL |