Detects 10 Rat Cytokines. Suitable for all liquid sample types.
Rat Cytokine Array Q2
Rat Cytokine Array Q2
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Rat |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
ICAM-1 (CD54)
| IFN-gamma
| IL-1 beta (IL-1 F2)
| IL-10
| IL-6
|
Leptin
| L-Selectin (CD62L)
| MCP-1 (CCL2)
| TIMP-1
| TNF alpha
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Rat Cytokine Array Q2 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAR-CYT-2.gal)
Excel (QAR-CYT-2_Analysis_Tool.xls)
citations
Open hepatic artery flow with portal vein clamping protects against bile duct injury compared to pringles maneuver
- Species:
- Rat
- Sample Type:
- bile
- Year:
- 2023
- Journal Name:
- Scandinavian Journal of Gastroenterology
Bifidobacterium longum alleviates irritable bowel syndrome-related visceral hypersensitivity and microbiota dysbiosis via Paneth cell regulation
- Species:
- Rat
- Sample Type:
- Serum (Probiotic rat IBS model)
- Year:
- 2020
- Journal Name:
- Gut microbes
- PubMed ID:
- 32584650
Investigating the effects of dexamethasone on blood-brain barrier permeability and inflammatory response following focused ultrasound and microbubble exposure
- Species:
- Rat
- Sample Type:
- Tissue Lysate
- Year:
- 2020
- Journal Name:
- Theranostics
The role of macrophages in the development of biliary injury in a lipopolysaccharide-aggravated hepatic ischaemia-reperfusion model.
- Species:
- Rat
- Sample Type:
- Bile (cholangiocytes)
- Year:
- 2017
- Journal Name:
- Biochimica et Biophysica Acta Molecular Basis of Disease
- PubMed ID:
- 28709962
Tumor progression is associated with increasing CD11b+ cells and CCL2 in Lewis rat sarcoma
- Species:
- Rat
- Sample Type:
- Serum (Lewis Rat sarcoma model)
- Year:
- 2015
- Journal Name:
- Anticancer Research
- PubMed ID:
- 25667449
Reversible and irreversible differentiation of cardiac fibroblasts
- Species:
- Rat
- Sample Type:
- Conditioned Media
- Year:
- 2014
- Journal Name:
- Cardiovascular Research
- PubMed ID:
- 24368833
Discovery and preclinical characterization of novel small molecule TRK and ROS1 tyrosine kinase inhibitors for the treatment of cancer and inflammation.
- Species:
- Rat
- Sample Type:
- Air Pouch Exudates
- Year:
- 2013
- Journal Name:
- Plos One
- PubMed ID:
- 24386191
Significant reduction of acute cardiac allograft rejection by selective janus kinase-1/3 inhibition using R507 and R545
- Species:
- Rat
- Sample Type:
- Tissue Lysate (Graft tissue lysate from heart tissue)
- Year:
- 2012
- Journal Name:
- Transplantation
- PubMed ID:
- 22971540