Reagents and instruments for immunology, cell biology and molecular biology.
 
> Recombinant Rat Epidermal Growth Factor (rRtEGF )

Recombinant Rat Epidermal Growth Factor (rRtEGF )

Print Print
Please log in

Cat# orb1495058-1mg

Size : 1mg

Brand : Biorbyt


Recombinant Rat Epidermal Growth Factor (rRtEGF )

SKU: orb1495058
This product can be custom made

Description

EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor).

Research Area

Stem Cell & Developmental Biology

Images & Validation

−
Recombinant Rat Epidermal Growth Factor (rRtEGF )
Application Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Key Properties

−
SourceEscherichia coli.
Biological ActivityFully biologically active when compared to standard. The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is 1 x 107 units/mg.
Purification97% by SDS-PAGE and HPLC analyses.
EndotoxinsLess than 1EU/mg of rRtEGF as determined by LAL method.
MW6.2 kDa, a single non-glycosylated polypeptide chain containing 54 amino acids, including 3 intramolecular disulfide-bonds.
Purity97% by SDS-PAGE and HPLC analyses.
Protein SequenceMNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR

Storage & Handling

−
StorageThis lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Form/AppearanceLyophilized from a 0.2mm filtered solution in PBS, pH 7.4.
Buffer/PreservativesLyophilized from a 0.2mm filtered solution in PBS, pH 7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide