Detection of cell contamination
Mycoplasma and bacterial contamination is a major concern for research laboratories (e.g. in cell biology) or biopharmaceutical laboratories (production of vaccines, monoclonal antibodies, etc.).
Mycoplasmas are the smallest self-producing microorganisms and can reproduce in extracellular space. Although they are mostly harmless to humans, other host animals and plants, some species can cause disease in humans. In particular, 6 species are a frequent cause of contamination in continuous cell lines and primary cells. Because of their size, mycoplasma are not detectable (e.g. under the microscope) and yet they profoundly alter cell cultures (metabolism, cell growth, pH, viability, membrane transport).
The main source of mycoplasma contamination is other infected cell cultures as well as culture media, reagents (fetal bovine serum, trypsin, etc.) and laboratory personnel.
Mycoplasma contaminations can cost money, time and reputation, compromise research results or impact the safety of biologic therapies. Effective strategies against mycoplasma contaminations are prevention, early detection, removal of infected material, or disposal of mycoplasma.
Early detection of bacteria in cell cultures is the only reasonable solution and the only control measure against a common problem for life science laboratories: bacterial contaminations in cell cultures.
Up to 5% of all cell cultures are contaminated with bacteria but show no visual signs of bacterial contamination, such as turbidity, sudden change in the colour of the medium (phenol red) or microscopic particles. In addition, bacteria may show resistance or lack of sensitivity to standard antibiotics (e.g. penicillin/streptomycin) and have a direct impact on cell metabolism, growth and differentiation.
Frequently Asked Questions (FAQ)
Where can I find detection products on the CliniSciences website?
detection products are available in this section and can be sorted by product families or sub-categories, such as Mycoplasma, Related reagents, Bacteria. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the detection category?
The detection category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The Mycoplasma, Related reagents, Bacteria sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a detection product?
To find a detection product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does CliniSciences stock several product codes in the detection category?
Yes, CliniSciences distributes several product references in the detection category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for detection products?
Yes, you can request a quote for detection products from the product page or by contacting CliniSciences (info@clinisciences.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a detection product is available to order?
The availability of a detection product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact CliniSciences with the product code or name of the product you are looking for.
What should I do if I cannot find the detection product I am looking for?
If the detection product you are looking for does not appear in the catalogue, please contact CliniSciences with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all detection products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.