Human whole blood is a complex biological matrix comprising plasma, erythrocytes, leukocytes, platelets, proteins, nucleic acids, metabolites, and extracellular vesicles. Human whole blood collected with EDTA (ethylenediaminetetraacetic acid) is widely used as a research biospecimen because EDTA chelates calcium ions required for coagulation, thereby maintaining the sample in an anticoagulated state for subsequent cellular and molecular analyses. Its diverse cellular and molecular composition makes whole blood useful for investigating systemic biological processes, circulating biomarkers, hematological characteristics, and molecular profiles.

Key Features

• EDTA-Anticoagulated Whole Blood : Provides a non-clotted blood matrix for downstream laboratory processing.

• Comprehensive Biological Matrix : Contains circulating blood cells, plasma constituents, nucleic acids, metabolites, proteins, and extracellular vesicles.

• Nucleic-Acid Analysis : Can be processed for genomic DNA and RNA isolation, depending on the collection method, processing interval, and storage conditions.

• Broad Research Utility : Supports molecular, cellular, immunological, genomic, transcriptomic, and hematological investigations.

Usual Research Uses

• Genomic DNA Extraction : Isolation of genomic DNA for PCR, qPCR, genotyping, sequencing, and other molecular analyses.

• RNA Isolation and Analysis : Recovery of RNA for gene-expression and transcriptomic studies, depending on collection, processing, and storage conditions.

• Leukocyte and PBMC Isolation : Recovery of circulating immune-cell populations for cellular, immunological, and functional studies.

• PCR and qPCR : Detection and quantification of nucleic-acid targets.

• Sequencing : Preparation of blood-derived nucleic acids for targeted and other sequencing workflows.

• Gene-Expression Studies : Investigation of transcriptional profiles in circulating blood cells.

• Biomarker Research : Analysis of circulating cellular and molecular biomarkers.

• Immunological Research : Characterization of circulating immune-cell populations and immune-related molecular features.

• Biobanking : Collection and storage of blood-derived cellular and molecular material for subsequent research applications.