PerCP-conjugated anti-guinea pig primary antibodies combine the specificity of anti-guinea pig immunoglobulin antibodies with the naturally occurring fluorophore Peridinin Chlorophyll Protein Complex (PerCP). Originally isolated from photosynthetic dinoflagellates, PerCP is characterized by strong excitation around 482 nm and bright far-red emission near 675 nm, making it ideally suited for multicolor fluorescence applications using standard 488 nm laser-equipped flow cytometers.

Conjugate Characteristics and Specificity

PerCP-conjugated anti-guinea pig antibodies are typically affinity-purified polyclonal antibodies produced in goat or rabbit hosts. Most preparations recognize both the heavy and light (H+L) chains of guinea pig IgG, while Fc-specific formats are also available to eliminate binding to F(ab')₂ fragments. Optimized conjugation preserves both antibody specificity and the fluorescence properties of PerCP, providing reliable signal intensity while minimizing steric hindrance and non-specific interactions.

Applications 

• Flow cytometry: Primarily used as a secondary detection reagent for immunophenotyping and multicolor flow cytometric analysis. PerCP is efficiently excited by the 488 nm laser and emits in the far-red region, allowing excellent spectral separation from fluorophores such as FITC and PE.
• Multiplex fluorescence assays: Suitable for immunofluorescence applications where its long-wavelength emission supports simultaneous detection of multiple targets with reduced spectral overlap.